DLL3 Antibody (SC16.15) - Chimeric - BSA Free

Novus Biologicals | Catalog # NBP3-45128

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat, Cynomolgus Monkey

Applications

ELISA, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # SC16.15

Format

BSA Free
View all DLL3 Primary Antibodies »

Product Specifications

Immunogen

Three strains of mice (Balb/c, CD-1 and FVB) were immunized with recombinant human DLL3-Fc or with human DLL3-His fusion constructs (Uniprot#: Q9NYJ7)

Specificity

This antibody binds human DLL3 in the N-terminal domain

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Scientific Data Images for DLL3 Antibody (SC16.15) - Chimeric - BSA Free

DLL3 Antibody (SC16.15) - Chimeric - BSA Free DLL3 Antibody (SC16.15) - Chimeric - BSA Free

Immunocytochemistry/ Immunofluorescence: DLL3 Antibody (SC16.15) - Chimeric - BSA Free [NBP3-45128] -

Immunocytochemistry/ Immunofluorescence: DLL3 Antibody (SC16.15) - Chimeric - BSA Free [NBP3-45128] - Immunofluorescence analysis of paraformaldehyde-fixed U2OS cells on coverslips stained with NBP3-45128 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic, vesicle, and nuclear staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-45128, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.

Applications for DLL3 Antibody (SC16.15) - Chimeric - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody can mediate killing of tumor cells expressing DLL3 and facilitate the delivery of cytotoxic agents to live cells, in an vitro cell killing assay. This antibody was capable of killing OV26 cells, an ovarian NET NTX tumor, with a sub-picomolar EC50 (e.g. 0.14 pM).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DLL3

The DLL3 gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in this gene cause autosomal recessive spondylocostal dysostos

Long Name

Delta-like 3

Alternate Names

Pudgy, SCDO1

Gene Symbol

DLL3

Additional DLL3 Products

Product Documents for DLL3 Antibody (SC16.15) - Chimeric - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for DLL3 Antibody (SC16.15) - Chimeric - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Notch Pathway

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Notch Signaling Pathways
Notch Signaling Pathway Thumbnail