DNA-PKcs Antibody (3H6) - BSA Free
Novus Biologicals | Catalog # NBP2-22128
![Western Blot: DNA-PKcs Antibody (3H6)BSA Free [NBP2-22128]](https://resources.rndsystems.com/images/products/DNA-PKcs-Antibody-3H6-Western-Blot-NBP2-22128-img0006.jpg "Western Blot: DNA-PKcs Antibody (3H6)BSA Free [NBP2-22128]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 3H6
Format
BSA Free
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Product Specifications
Immunogen
Purified recombinant fragment of human DNA PKcs expressed in E. coli. [Uniprot: P78527]
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
460 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DNA-PKcs Antibody (3H6) - BSA Free
Western Blot: DNA-PKcs Antibody (3H6)BSA Free [NBP2-22128]
Western Blot: DNA-PKcs Antibody (3H6) [NBP2-22128] - HeLa (A), MCF7 (lB) and HEK293 (C) whole cell protein was separated by SDS-PAGE on 6% gel, transferred to PVDF and probed with NBP2-22128. A single band of DNA PKcs reactivity at approximately 460 kDa was detected in all lysates.![Immunocytochemistry/ Immunofluorescence: DNA-PKcs Antibody (3H6) - BSA Free [NBP2-22128]](https://resources.rndsystems.com/images/products/DNA-PKcs-Antibody-3H6-Immunocytochemistry-Immunofluorescence-NBP2-22128-img0003.jpg "Immunocytochemistry/ Immunofluorescence: DNA-PKcs Antibody (3H6) - BSA Free [NBP2-22128]")
Immunocytochemistry/ Immunofluorescence: DNA-PKcs Antibody (3H6) - BSA Free [NBP2-22128]
Immunocytochemistry/Immunofluorescence: DNA-PKcs Antibody (3H6) [NBP2-22128] - Analysis of Hela cells using DNA PKcs mouse mAb (green). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).![Immunohistochemistry-Paraffin: DNA-PKcs Antibody (3H6) - BSA Free [NBP2-22128]](https://resources.rndsystems.com/images/products/DNA-PKcs-Antibody-3H6-Immunohistochemistry-Paraffin-NBP2-22128-img0005.jpg "Immunohistochemistry-Paraffin: DNA-PKcs Antibody (3H6) - BSA Free [NBP2-22128]")
Immunohistochemistry-Paraffin: DNA-PKcs Antibody (3H6) - BSA Free [NBP2-22128]
Immunohistochemistry-Paraffin: DNA-PKcs Antibody (3H6) [NBP2-22128] - Analysis of paraffin-embedded lung cancer (left) and brain tissues (right) using DNA PKcs mouse mAb with DAB staining.![Western Blot: DNA-PKcs Antibody (3H6)BSA Free [NBP2-22128]](https://resources.rndsystems.com/images/products/DNA-PKcs-Antibody-3H6-Western-Blot-NBP2-22128-img0002.jpg "Western Blot: DNA-PKcs Antibody (3H6)BSA Free [NBP2-22128]")
Western Blot: DNA-PKcs Antibody (3H6)BSA Free [NBP2-22128]
Western Blot: DNA-PKcs Antibody (3H6) [NBP2-22128] - Analysis using DNA PKcs mAb against HEK293 (1) and DNA PKcs(AA: 2638-2971)-hIgGFc transfected HEK293 (2) cell lysates.![Immunohistochemistry-Paraffin: DNA-PKcs Antibody (3H6) - BSA Free [NBP2-22128]](https://resources.rndsystems.com/images/products/DNA-PKcs-Antibody-3H6-Immunohistochemistry-Paraffin-NBP2-22128-img0004.jpg "Immunohistochemistry-Paraffin: DNA-PKcs Antibody (3H6) - BSA Free [NBP2-22128]")
Immunohistochemistry-Paraffin: DNA-PKcs Antibody (3H6) - BSA Free [NBP2-22128]
Immunohistochemistry-Paraffin: DNA-PKcs Antibody (3H6) [NBP2-22128] - Analysis of paraffin-embedded breast cancer (left) and colon cancer (right) using DNA PKcs mouse mAb with DAB staining.![ELISA: DNA-PKcs Antibody (3H6) - BSA Free [NBP2-22128]](https://resources.rndsystems.com/images/products/DNA-PKcs-Antibody-3H6-ELISA-NBP2-22128-img0001.jpg "ELISA: DNA-PKcs Antibody (3H6) - BSA Free [NBP2-22128]")
ELISA: DNA-PKcs Antibody (3H6) - BSA Free [NBP2-22128]
ELISA: DNA-PKcs Antibody (3H6) [NBP2-22128] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).Applications for DNA-PKcs Antibody (3H6) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Immunocytochemistry/ Immunofluorescence
1:200-1:1000
Immunohistochemistry-Paraffin
1:200-1:1000
Western Blot
1:500-1:2000
Application Notes
In Western blot, a band can be seen at 460-470 kDa representing DNA PKcs.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DNA-PKcs
Long Name
DNA-dependent Protein Kinase Catalytic Subunit
Alternate Names
DNAPK, DNAPKcs, DNPK1, HYRC1, p350, PRKDC, XRCC7
Entrez Gene IDs
5591 (Human)
Gene Symbol
PRKDC
UniProt
Additional DNA-PKcs Products
Product Documents for DNA-PKcs Antibody (3H6) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for DNA-PKcs Antibody (3H6) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for DNA-PKcs Antibody (3H6) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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