DNA-PKcs Antibody

Novus Biologicals | Catalog # NB100-658

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation, Knockdown Validated

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all DNA-PKcs Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 2050 and 2100 of human DNA-Dependent Protein Kinase, catalytic subunit using the numbering given in entry NP_008835.5 (GeneID 5591).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNA-PKcs Antibody

Western Blot: DNA-PKcs Antibody [NB100-658]

Western Blot: DNA-PKcs Antibody [NB100-658]

Western Blot: DNA-PKcs Antibody [NB100-658] - MDA-MB-231, MCF-7, SUM149, SUM159, T47D, HCC1954 whole cell lysates (50 ug/lane). 10% SDS-PAGE. DNA-PKcs (NB100-658) primary antibody at 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.
Western Blot: DNA-PKcs Antibody [NB100-658]

Western Blot: DNA-PKcs Antibody [NB100-658]

Western Blot: DNA-PKcs Antibody [NB100-658] - Detection of Human DNA-PKcs in HeLa whole cell lystate using NB100-658. DNA-PKcs was also immunoprecipitated using rabbit anti-DNA-PKcs antibodies NB100-657, NB100-659 and NB100-660.
Western Blot: DNA-PKcs Antibody [NB100-658]

Western Blot: DNA-PKcs Antibody [NB100-658]

Western Blot: DNA-PKcs Antibody [NB100-658] - Hep3B human hepatocellular carcinoma cell line. 50 ug cell lysate per lane. 10% SDS-PAGE. DNA-PKcs antibody at 1:1000 dilution O/N. Secondary antibody: HRP-conjugated Donkey anti-rabbit IgG polyclonal antibody at 1:2000 dilution. WB image submitted by a verified customer review.
Knockdown Validated: DNA-PKcs Antibody [NB100-658]

Western Blot: DNA-PKcs Antibody [NB100-658]

DNA-PKcs-Antibody-Knockdown-Validated-NB100-658-img0026.jpg
DNA-PKcs Antibody

Western Blot: DNA-PKcs Antibody [NB100-658] -

Western Blot: DNA-PKcs Antibody [NB100-658] - Molecular Characterization of G1 Resection(A) Schematic of the NHEJ reporter assay. The repair of two I-SceI-induced DSBs can result in loss of the intervening fragment, which is detected by a CD4+ signal (Rass et al., 2009). CD4+ clones were amplified by PCR (green arrows) across the repair site & sequenced. Repair of the two DSBs can also occur without loss of the intervening fragment, which escapes detection.(B) gamma H2AX foci in GC92 WT & Artemis KO cells treated with siDNA-PKcs or siCtIP. Cells were transfected with I-SceI, & foci were scored in I-SceI+ & I-SceI− cells (identified by immunofluorescence [IF] against I-SceI). Data are mean ± SEM.(C) End joining events in GC92 WT & Artemis KO cells containing the NHEJ reporter substrate. Cells were transfected with RFP or cMyc-Artemis constructs. Events were quantified by the fraction of CD4+ & RFP/cMyc+cells relative to all RFP/cMyc+cells, & results were normalized to WT cells. Data are mean ± SEM.(D) End joining events in GC92 WT & Artemis KO cells treated with siCtIP. Data are mean ± SEM.(E) Distribution of deletion sizes obtained from the sequence analysis of GC92 WT & siLig1/3-treated cells. nt, nucleotide.(F) End joining events in GC92 cells treated with siKu70/80, siLig4, siLig1/3, or siDNA-PKcs. Data are mean ± SEM.See also Table S1. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28132842), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for DNA-PKcs Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:1000 - 1:10000
Application Notes
Ku-80 co-immunoprecipitates with DNA-PKcs when using this antibody. DNA-PKcs antibody is validated for WB from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NB100-658 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS, 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DNA-PKcs

DNA PK is the key component of the non-homologous end-joining (NHEJ) pathway of DSB repair in mammalian cells. DNA PK consists of a heterodimeric DNA-binding subunit (Ku70/80) and an approximately 465 kDa catalytic subunit (DNA PKcs). DNA PKcs is a serine/threonine protein kinase whose activity is greatly stimulated by its recruitment to DNA breaks by the Ku heterodimer.

Long Name

DNA-dependent Protein Kinase Catalytic Subunit

Alternate Names

DNAPK, DNAPKcs, DNPK1, HYRC1, p350, PRKDC, XRCC7

Entrez Gene IDs

5591 (Human)

Gene Symbol

PRKDC

UniProt

P78527

Additional DNA-PKcs Products

Product Documents for DNA-PKcs Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for DNA-PKcs Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

DNA Repair

Citations for DNA-PKcs Antibody

Customer Reviews for DNA-PKcs Antibody (1)

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  • DNA-PKcs Antibody
    Name: Yongkang Yang
    Application: Western Blot
    Sample Tested: Hep3B human hepatocellular carcinoma cell line
    Species: Human
    Verified Customer | Posted 08/16/2019
    50ug Hep3b cells lysate/lane, 10% SDS-PAGE; Primary antibody (DNA-PKcs Antibody (NB100-658), Lot: A1 ): 1:1000 diluted in 5% milk incubate at 4℃ for overnight; Secondary antibody: NA934V, Donkey Anti-Rabbit IgG Polyclonal Antibody (HRP (Horseradish Peroxidase)-  GE Healthcare (Lot: 16908227) (1:2000 diluted in 5% milk). Incubate at room temperature for 1 hour.
    DNA-PKcs Antibody NB100-658

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