DNA-PKcs [p Ser2056] Antibody

Novus Biologicals | Catalog # NBP3-13142

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all DNA-PKcs Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser2056 of human DNA-PKcs. The exact sequence is proprietary.

Modification

p Ser 2056

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for DNA-PKcs [p Ser2056] Antibody

Western Blot: DNA-PKcs [p Ser2056] Antibody [NBP3-13142]

Western Blot: DNA-PKcs [p Ser2056] Antibody [NBP3-13142]

Western Blot: DNA-PKcs [p Ser2056] Antibody [NBP3-13142] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA-PKcs (phospho Ser2056) antibody (NBP3-13142) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: DNA-PKcs [p Ser2056] Antibody [NBP3-13142]

Immunocytochemistry/ Immunofluorescence: DNA-PKcs [p Ser2056] Antibody [NBP3-13142]

Immunocytochemistry/Immunofluorescence: DNA-PKcs [p Ser2056] Antibody [NBP3-13142] - DNA-PKcs (phospho Ser2056) antibody detects DNA-PKcs (phospho Ser2056) protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNA-PKcs (phospho Ser2056) stained by DNA-PKcs (phospho Ser2056) antibody (NBP3-13142) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.
Western Blot: DNA-PKcs [p Ser2056] Antibody [NBP3-13142]

Western Blot: DNA-PKcs [p Ser2056] Antibody [NBP3-13142]

Western Blot: DNA-PKcs [p Ser2056] Antibody [NBP3-13142] - Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA-PKcs (phospho Ser2056) antibody (NBP3-13142) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for DNA-PKcs [p Ser2056] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DNA-PKcs

The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.(supplied by OMIM)

Long Name

DNA-dependent Protein Kinase Catalytic Subunit

Alternate Names

DNAPK, DNAPKcs, DNPK1, HYRC1, p350, PRKDC, XRCC7

Gene Symbol

PRKDC

Additional DNA-PKcs Products

Product Documents for DNA-PKcs [p Ser2056] Antibody

Certificate of Analysis

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Product Specific Notices for DNA-PKcs [p Ser2056] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

DNA Repair

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Protocols

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FAQs

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