DRP1 Antibody (4A4C7)

Novus Biologicals | Catalog # NBP2-37309

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4A4C7
View all DRP1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human DRP1 (AA: 69-213) expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

81.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DRP1 Antibody (4A4C7)

Western Blot: DRP1 Antibody (4A4C7)BSA Free [NBP2-37309]

Western Blot: DRP1 Antibody (4A4C7)BSA Free [NBP2-37309]

Western Blot: DRP1 Antibody (4A4C7) [NBP2-37309] - Analysis using DNM1L mouse mAb against PC-12 (1), LNCAP (2) and NIH/3T3 (3) cell lysate.
Immunocytochemistry/ Immunofluorescence: DRP1 Antibody (4A4C7) - BSA Free [NBP2-37309]

Immunocytochemistry/ Immunofluorescence: DRP1 Antibody (4A4C7) - BSA Free [NBP2-37309]

Immunocytochemistry/Immunofluorescence: DRP1 Antibody (4A4C7) [NBP2-37309] - Analysis of Hela cells using DNM1L mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry: DRP1 Antibody (4A4C7) - BSA Free [NBP2-37309]

Flow Cytometry: DRP1 Antibody (4A4C7) - BSA Free [NBP2-37309]

Flow Cytometry: DRP1 Antibody (4A4C7) [NBP2-37309] - Analysis of HEK293 cells using DNM1L mouse mAb (green) and negative control (red).
Western Blot: DRP1 Antibody (4A4C7)BSA Free [NBP2-37309]

Western Blot: DRP1 Antibody (4A4C7)BSA Free [NBP2-37309]

Western Blot: DRP1 Antibody (4A4C7) [NBP2-37309] - Analysis using DNM1L mAb against human DNM1L recombinant protein. (Expected MW is 41.8 kDa)
Western Blot: DRP1 Antibody (4A4C7)BSA Free [NBP2-37309]

Western Blot: DRP1 Antibody (4A4C7)BSA Free [NBP2-37309]

Western Blot: DRP1 Antibody (4A4C7) [NBP2-37309] - Analysis using DNM1L mAb against HEK293 (1) and DNM1L (AA: 69-213)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: DRP1 Antibody (4A4C7) - BSA Free [NBP2-37309]

ELISA: DRP1 Antibody (4A4C7) - BSA Free [NBP2-37309]

ELISA: DRP1 Antibody (4A4C7) [NBP2-37309] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for DRP1 Antibody (4A4C7)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Western Blot

1:500 - 1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DRP1

DRP1 is a member of the dynamin GTPase superfamily, which that contributes to mitochondrial division in mammalian cells. DRP1 plays this important role in mitochondrial fission at steady state and during apoptosis. DRP1 is required for proper cellular distribution of mitochondria, and in mutant neurons, mitochondria are largely absent from synapses. Therefore, DRP1 provides a genetic tool to assess the role of mitochondria at synapses.

The DRP1 gene has 3 alternatively spliced transcripts encoding different isoforms, which are alternatively polyadenylated.

Long Name

Dynamin-1-like protein

Alternate Names

DLP1, DNM1L, DVLP, Dymple, HdynIV

Gene Symbol

DNM1L

Additional DRP1 Products

Product Documents for DRP1 Antibody (4A4C7)

Certificate of Analysis

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Download SDS

Product Specific Notices for DRP1 Antibody (4A4C7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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