E-Tag Epitope Tag Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-43762

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Epitope Tag

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all E-Tag Epitope Tag Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the E-Tag Epitope Tag sequence (GAPVPYPDPLEPR)

Specificity

Recognizes N-terminal and C-terminal E-Tag Epitope Tag in fusion proteins

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for E-Tag Epitope Tag Antibody - Azide and BSA Free

E-Tag Epitope Tag Antibody - Azide and BSA Free

Western Blot: E-Tag Epitope Tag Antibody [NBP3-43762] -

Western Blot: E-Tag Epitope Tag Antibody [NBP3-43762] - NBP3-43762 at 1:1000 dilution. Lane A: Etag-GFERh(C) transfected 293 cell lysate at 2ug Lane B: Nagetive control at 10ug Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions.
E-Tag Epitope Tag Antibody - Azide and BSA Free

Immunoprecipitation: E-Tag Epitope Tag Antibody [NBP3-43762] -

Immunoprecipitation: E-Tag Epitope Tag Antibody [NBP3-43762] - E-tag was immunoprecipitated using: Lane A:0.5 mg E-tag Whole Cell Lysate 4 uL NBP3-43762 and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: NBP3-43762, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 17 kDa Observed band size :17 kDa

Applications for E-Tag Epitope Tag Antibody - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

1-5 uL/mg lysate

Western Blot

1:1000-1:5000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: E-Tag Epitope Tag

The E-Tag is a popular epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized E-tag epitope represents the peptide sequence GAPVPYPDPLEPR.

Alternate Names

E epitope tag, GAPVPYPDPLEPR epitope tag, GAPVPYPDPLEPR tag

Additional E-Tag Epitope Tag Products

Product Documents for E-Tag Epitope Tag Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for E-Tag Epitope Tag Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for E-Tag Epitope Tag Antibody - Azide and BSA Free

There are currently no reviews for this product. Be the first to review E-Tag Epitope Tag Antibody - Azide and BSA Free and earn rewards!

Have you used E-Tag Epitope Tag Antibody - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies