E2F6 Antibody (9I5M0)

Novus Biologicals | Catalog # NBP3-15400

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9I5M0 expressed in HEK293
View all E2F6 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 110-281 of human E2F6 (NP_937987.2) LDGIDLVEKKSKNHIRWIGSDLSNFGAVPQQKKLQEELSDLSAMEDALDELIKDCAQQLFELTDDKENERLAYVTYQDIHSIQAFHEQIVIAVKAPAETRLDVPAPREDSITVHIRSTNGPIDVYLCEVEQGQTSNKRSEGVGTSSSESTHPEGPEEEENPQQSEELLEVSN

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for E2F6 Antibody (9I5M0)

Western Blot: E2F6 Antibody (9I5M0) [NBP3-15400]

Western Blot: E2F6 Antibody (9I5M0) [NBP3-15400]

Western Blot: E2F6 Antibody (9I5M0) [NBP3-15400] - Western blot analysis of extracts of various cell lines, using E2F6 Rabbit mAb (NBP3-15400) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Chromatin Immunoprecipitation: E2F6 Antibody (9I5M0) [NBP3-15400] - Chromatin immunoprecipitation analysis of extracts of 293T cells, using E2F6 antibody (NBP3-15400) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Immunoprecipitation: E2F6 Antibody (9I5M0) [NBP3-15400]

Immunoprecipitation: E2F6 Antibody (9I5M0) [NBP3-15400]

Immunoprecipitation: E2F6 Antibody (9I5M0) [NBP3-15400] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug E2F6 antibody (NBP3-15400). Western blot was performed from the immunoprecipitate using E2F6 antibody (NBP3-15400) at a dilition of 1:1000.

Applications for E2F6 Antibody (9I5M0)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:500 - 1:1000

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: E2F6

E2F6 encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms.

Alternate Names

E2F transcription factor 6, E2F-6MGC111545, transcription factor E2F6

Gene Symbol

E2F6

Additional E2F6 Products

Product Documents for E2F6 Antibody (9I5M0)

Certificate of Analysis

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Product Specific Notices for E2F6 Antibody (9I5M0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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