EEA1 Antibody (6D4.1D4) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80711

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow (Intracellular)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6D4.1D4

Format

Azide and BSA Free
View all EEA1 Primary Antibodies »

Product Specifications

Immunogen

Partial recombinant human EEA1 protein (between amino acids 50-300). [UniProt Q15075]

Localization

Cytoplasm. Early endosome membrane.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for EEA1 Antibody (6D4.1D4) - Azide and BSA Free

Western Blot: EEA1 Antibody (6D4.1D4)Azide and BSA Free [NBP2-80711]

Western Blot: EEA1 Antibody (6D4.1D4)Azide and BSA Free [NBP2-80711]

Western Blot: EEA1 Antibody (6D4.1D4) - Azide and BSA Free [NBP2-80711] - Analysis of partial recombinant human EEA1 protein (14kDa) with EEA1 antibody clone 6D4.1D4 at 0.5 ug mL. Image from the standard format of this antibody.
Immunohistochemistry: EEA1 Antibody (6D4.1D4) - Azide and BSA Free [NBP2-80711]

Immunohistochemistry: EEA1 Antibody (6D4.1D4) - Azide and BSA Free [NBP2-80711]

Immunohistochemistry: EEA1 Antibody (6D4.1D4) - Azide and BSA Free [NBP2-80711] - Analysis of FFPE tissue section of human testes using mouse monoclonal EEA1 antibody (clone 6D4.1D4) at 10 ug/ml concentration. The interstitial cells depicted a punctate to granular cytoplasmic staining pattern for EEA1 protein. Image from the standard f
Immunohistochemistry-Paraffin: EEA1 Antibody (6D4.1D4) - Azide and BSA Free [NBP2-80711]

Immunohistochemistry-Paraffin: EEA1 Antibody (6D4.1D4) - Azide and BSA Free [NBP2-80711]

Immunohistochemistry-Paraffin: EEA1 Antibody (6D4.1D4) - Azide and BSA Free [NBP2-80711] - Analysis of FFPE tissue section of mouse testes using mouse monoclonal EEA1 antibody (clone 6D4.1D4) at 10 ug/ml concentration. The interstitial cells depicted a punctate to granular cytoplasmic staining pattern for EEA1 protein with no signal in other ce

Applications for EEA1 Antibody (6D4.1D4) - Azide and BSA Free

Application
Recommended Usage

Flow (Intracellular)

2.5 ug/million cells

Immunohistochemistry

5-10 ug/ml

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EEA1

Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc finger like domains, reminiscent of those found in nucleic acid binding proteins, are located in the amino and carboxyl terminal domains of EEA1. The carboxyl terminal zinc finger like domain is conserved in several other non nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown dependent on phosphatidyl 3 kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes.

Long Name

Early Endosome Antigen 1

Alternate Names

MST105, MSTP105, ZFYVE2

Gene Symbol

EEA1

Additional EEA1 Products

Product Documents for EEA1 Antibody (6D4.1D4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for EEA1 Antibody (6D4.1D4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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