EEA1 Antibody (GT10811) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-13599
![Western Blot: EEA1 Antibody (GT10811) [NBP3-13599]](https://resources.rndsystems.com/images/products/EEA1-Antibody-GT10811-Western-Blot-NBP3-13599-img0001.jpg "Western Blot: EEA1 Antibody (GT10811) [NBP3-13599]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # GT10811
Format
Azide and BSA Free
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Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EEA1. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
162 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for EEA1 Antibody (GT10811) - Azide and BSA Free
Western Blot: EEA1 Antibody (GT10811) [NBP3-13599]
Western Blot: EEA1 Antibody (GT10811) [NBP3-13599] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EEA1 antibody [GT10811] (NBP3-13599) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.![Immunohistochemistry-Paraffin: EEA1 Antibody (GT10811) [NBP3-13599]](https://resources.rndsystems.com/images/products/EEA1-Antibody-GT10811-Immunohistochemistry-Paraffin-NBP3-13599-img0003.jpg "Immunohistochemistry-Paraffin: EEA1 Antibody (GT10811) [NBP3-13599]")
Immunohistochemistry-Paraffin: EEA1 Antibody (GT10811) [NBP3-13599]
Immunohistochemistry-Paraffin: EEA1 Antibody (GT10811) [NBP3-13599] - EEA1 antibody [GT10811] detects EEA1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. EEA1 stained by EEA1 antibody [GT10811] (NBP3-13599) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min![Immunohistochemistry-Paraffin: EEA1 Antibody (GT10811) [NBP3-13599]](https://resources.rndsystems.com/images/products/EEA1-Antibody-GT10811-Immunohistochemistry-Paraffin-NBP3-13599-img0002.jpg "Immunohistochemistry-Paraffin: EEA1 Antibody (GT10811) [NBP3-13599]")
Immunohistochemistry-Paraffin: EEA1 Antibody (GT10811) [NBP3-13599]
Immunohistochemistry-Paraffin: EEA1 Antibody (GT10811) [NBP3-13599] - EEA1 antibody [GT10811] detects EEA1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. EEA1 stained by EEA1 antibody [GT10811] (NBP3-13599) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/ Immunofluorescence: EEA1 Antibody (GT10811) [NBP3-13599] -
EEA1 antibody [GT10811] detects EEA1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EEA1 stained by EEA1 antibody [GT10811] (NBP3-13599) diluted at 1:500.
Blue: Hoechst 33342 staining.
Applications for EEA1 Antibody (GT10811) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:1000-1:10000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: EEA1
Long Name
Early Endosome Antigen 1
Alternate Names
MST105, MSTP105, ZFYVE2
Gene Symbol
EEA1
Additional EEA1 Products
Product Documents for EEA1 Antibody (GT10811) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for EEA1 Antibody (GT10811) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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