EEA1 Antibody

Novus Biologicals | Catalog # NBP1-05962

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Sheep

Cited:

Human, Mouse, Ovine

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all EEA1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 175 and 225 of human early endosome antigen 1 using the numbering given in entry NP_003557.2 (GeneID 8411).

Reactivity Notes

Sheep reactivity reported in scientific literature (PMID: 30016419).

Marker

Early Endosome Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EEA1 Antibody

Western Blot: EEA1 Antibody [NBP1-05962]

Western Blot: EEA1 Antibody [NBP1-05962]

Western Blot: EEA1 Antibody [NBP1-05962] - Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells. EEA1 was also immunoprecipitated by rabbit anti-EEA1 antibodies NBP1-05961 and NBP1-05963.

Applications for EEA1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
ICC/IF reactivity reported in (PMID: 30016419).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: EEA1

Early endosomal autoantigen 1 (EEA1) bears an FYVE (Fab1p-YOPB-Vps27p-EEA1) domain which is an approximately 80 amino acid double-zinc binding motif that binds to PtdIns(3)P (phosphatidylinositol 3-phosphate). The FYVE domain is found in proteins involved in intracellular signaling, membrane trafficking, and cytoskeletal rearrangement. EEA1 binds to early endosomal membranes that contain PtdIns(3)P and plays a role in the docking and fusion of early endosomes. Alternative names for EEA1 include endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2, and ZFYVE2.

Long Name

Early Endosome Antigen 1

Alternate Names

MST105, MSTP105, ZFYVE2

Entrez Gene IDs

8411 (Human); 216238 (Mouse)

Gene Symbol

EEA1

UniProt

Q15075 (Human)

Additional EEA1 Products

Product Documents for EEA1 Antibody

Certificate of Analysis

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Product Specific Notices for EEA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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