EGLN2/PHD1 Antibody (24GB3910)

Novus Biologicals | Catalog # NBP3-50757

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB3910
View all EGLN2/PHD1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human EGLN2/PHD1 (Uniprot: Q96KS0)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EGLN2/PHD1 Antibody (24GB3910)

EGLN2/PHD1 Antibody (24GB3910)

Immunocytochemistry/ Immunofluorescence: EGLN2/PHD1 Antibody (24GB3910)[NBP3-50757] -

Immunocytochemical staining of HepG2 cells with EGLN2/PHD1 antibody (1:1,000). Nuclei were stained blue with DAPI; EGLN2/PHD1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
EGLN2/PHD1 Antibody (24GB3910)

Flow Cytometry: EGLN2/PHD1 Antibody (24GB3910)[NBP3-50757] -

Flow cytometric analysis of EGLN2/PHD1expression in HepG2 cells using EGLN2/PHD1 antibody (1:2,000). Green, isotype control; red, EGLN2.
EGLN2/PHD1 Antibody (24GB3910)

Western Blot: EGLN2/PHD1 Antibody (24GB3910)[NBP3-50757] -

Western blotting analysis using EGLN2/PHD1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for EGLN2/PHD1 Antibody (24GB3910)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EGLN2/PHD1

HIF prolyl hydroxylase 1 (HPH-3 or EGLN2) is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis.

Long Name

Egl Nine Homolog 2/Prolyl Hydroxylase Domain-containing Protein 1

Alternate Names

EIT6, HIFPH1, HPH-1, HPH-3, PHD1

Gene Symbol

EGLN2

Additional EGLN2/PHD1 Products

Product Documents for EGLN2/PHD1 Antibody (24GB3910)

Certificate of Analysis

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Product Specific Notices for EGLN2/PHD1 Antibody (24GB3910)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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