EGR2 Antibody (OTI1B12)

Novus Biologicals | Catalog # NBP2-45696

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1B12
View all EGR2 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 156-476 of human EGR2(NP_000390) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

50.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EGR2 Antibody (OTI1B12)

Western Blot: EGR2 Antibody (OTI1B12) [NBP2-45696]

Western Blot: EGR2 Antibody (OTI1B12) [NBP2-45696]

Western Blot: EGR2 Antibody (OTI1B12) [NBP2-45696] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EGR2.
Immunocytochemistry/ Immunofluorescence: EGR2 Antibody (OTI1B12) [NBP2-45696]

Immunocytochemistry/ Immunofluorescence: EGR2 Antibody (OTI1B12) [NBP2-45696]

Immunocytochemistry/Immunofluorescence: EGR2 Antibody (OTI1B12) [NBP2-45696] - Analysis of HeLa cells.
Immunohistochemistry: EGR2 Antibody (OTI1B12) [NBP2-45696]

Immunohistochemistry: EGR2 Antibody (OTI1B12) [NBP2-45696]

Immunohistochemistry: EGR2 Antibody (OTI1B12) [NBP2-45696] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunocytochemistry/ Immunofluorescence: EGR2 Antibody (OTI1B12) [NBP2-45696]

Immunocytochemistry/ Immunofluorescence: EGR2 Antibody (OTI1B12) [NBP2-45696]

Immunocytochemistry/Immunofluorescence: EGR2 Antibody (OTI1B12) [NBP2-45696] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY EGR2.

Applications for EGR2 Antibody (OTI1B12)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:150

Western Blot

1:500

Reviewed Applications

Read 1 review rated 1 using NBP2-45696 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EGR2

Early Growth Response 2 (EGR2) is an early growth response family member which binds to specific areas of DNA and helps control the activity of particular genes. Specifically, EGR2 activates several genes that are involved in the formation and maintenance of myelin, the fatty substance that covers and protects nerve cells.

Defects in EGR2 have been linked to a number of diseases including congenital hypomyelination neuropathy, Charcot-Marie-Tooth disease type 1D, and Dejerine-Sottas neuropathy. EGR 2 antibodies may be useful tools for nerurscience research.

Alternate Names

AT591, CMT1D, CMT4E, early growth response 2, early growth response 2 (Krox-20 homolog, Drosophila), early growth response protein 2, EGR-2, FLJ14547, Krox-20 homolog, Drosophila, KROX-20, Drosophila, homolog (early growth response-2), KROX20DKFZp686J1957, Zinc finger protein Krox-20

Gene Symbol

EGR2

Additional EGR2 Products

Product Documents for EGR2 Antibody (OTI1B12)

Certificate of Analysis

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Product Specific Notices for EGR2 Antibody (OTI1B12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for EGR2 Antibody (OTI1B12) (1)

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Customer Images

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  • EGR2 Antibody (OTI1B12)
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Mouse Ovary
    Species: Mouse
    Verified Customer | Posted 07/07/2021
    Testing of EGR2 (NBP2-45696) antibody on fresh-frozen, paraformaldehyde fixed sections of 18 days old fetal mouse ovaries. EGR2 positive signal was localized in the cytoplasm instead of nucleus of all cells.
    Fresh frozen sections of ovaries were fixed for 10 min in freshly made 4% paraformaldehyde. After blocking with 10% donkey serum, sections were incubated overnight with 1:100 dilution of EGR2 (NBP2-45695) antibody or mouse IgG (antibody control) and exposed 1h with donkey-anti mouse Alexa 594 plus DAPI. Mounted sections were imaged in a Leica confocal microscope.
    EGR2 Antibody (OTI1B12) NBP2-45696
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

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