ENPP-4 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-05178

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all ENPP-4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 90-230 of human ENPP4 (NP_055751.1). ANSMYDAVTKKHFSDSNDKDPFWWNEAVPIWVTNQLQENRSSAAAMWPGTDVPIHDTISSYFMNYNSSVSFEERLNNITMWLNNSNPPVTFATLYWEEPDASGHKYGPEDKENMSRVLKKIDDLIGDLVQRLKMLGLWENL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ENPP-4 Antibody - Azide and BSA Free

Western Blot: ENPP-4 AntibodyAzide and BSA Free [NBP3-05178]

Western Blot: ENPP-4 AntibodyAzide and BSA Free [NBP3-05178]

Western Blot: ENPP4 Antibody [NBP3-05178] - Analysis of extracts of various cell lines, using ENPP4 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit

Applications for ENPP-4 Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ENPP-4

ENPP-4 (Ectonucleotide pyrophosphatase/phosphodiesterase 4; NPP4) belongs to a group of ecto-enzymes which regulate the availability of extracellular nucleotides. ENPP-4 hydrolyzes phosphodiester bonds in nucleotides with a preference for adenine nucleotides. It cleaves the diadenosine compounds Ap3A and Ap4a which are released from the dense granules of thrombin-activated platelets. These reactions generate AMP and ADP from Ap3A cleavage, and AMP and ATP from Ap4A cleavage. ENPP-4 is expressed on the surface of vascular endothelial cells where its activity prolongs platelet aggretation and contributes to thrombus formation.

Long Name

Ectonucleotide Pyrophosphatase/Phosphodiesterase 4

Alternate Names

AP3Aase, ENPP4, NPP-4

Gene Symbol

ENPP4

Additional ENPP-4 Products

Product Documents for ENPP-4 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ENPP-4 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Related Research Areas

Coagulation
Phosphatases and Regulators

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