Enterovirus 71 VP1 Antibody (HL1929) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25451

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Virus

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1929 expressed in HEK293

Format

Azide and BSA Free
View all Enterovirus 71 VP1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide of Enterovirus 71 VP1

Reactivity Notes

Enterovirus 71

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Enterovirus 71 VP1 Antibody (HL1929) - Azide and BSA Free

Enterovirus 71 VP1 Antibody (HL1929) - Azide and BSA Free

Western Blot: Enterovirus 71 VP1 Antibody (HL1929) - Azide and BSA Free [NBP3-25451] -

Western Blot: Enterovirus 71 VP1 Antibody (HL1929) - Azide and BSA Free [NBP3-25451] - Non-infected (-) and infected (+) RD whole cell extracts (20 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [HL1929] (NBP3-25451) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Enterovirus 71 VP1 Antibody (HL1929) - Azide and BSA Free

ELISA: Enterovirus 71 VP1 Antibody (HL1929) - Azide and BSA Free [NBP3-25451] -

ELISA: Enterovirus 71 VP1 Antibody (HL1929) - Azide and BSA Free [NBP3-25451] - Indirect ELISA analysis was performed by coating the plate with recombinant full-length enterovirus 71 VP1 (4000-62.5 ng/mL). Coated protein was probed with Enterovirus 71 VP1 antibody [HL1929] (NBP3-25451) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.
Enterovirus 71 VP1 Antibody (HL1929) - Azide and BSA Free

ELISA: Enterovirus 71 VP1 Antibody (HL1929) - Azide and BSA Free [NBP3-25451] -

ELISA: Enterovirus 71 VP1 Antibody (HL1929) - Azide and BSA Free [NBP3-25451] - Sandwich ELISA detection of recombinant full-length Enterovirus 71 VP1 protein using antibodies as below. Capture: Enterovirus 71 VP1 antibody [HL1929] (NBP3-25451) (5 ug/mL) Detection: HRP-conjugated Enterovirus 71 VP1 antibody [HL1928] (NBP3-25450) (1 ug/mL) Please notice that NBP3-25450 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25451. Please contact us for custom HRP-conjugated antibody.
Enterovirus 71 VP1 Antibody (HL1929) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Enterovirus 71 VP1 Antibody (HL1929) - Azide and BSA Free [NBP3-25451] -

Immunocytochemistry/Immunofluorescence: Enterovirus 71 VP1 Antibody (HL1929) - Azide and BSA Free [NBP3-25451] - Enterovirus 71 VP1 antibody [HL1929] detects Enterovirus 71 VP1 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Enterovirus 71 VP1 stained by Enterovirus 71 VP1 antibody [HL1929] (NBP3-25451) diluted at 1:500. Blue: Fluoroshield with DAPI.
Enterovirus 71 VP1 Antibody (HL1929) - Azide and BSA Free

Western Blot: Enterovirus 71 VP1 Antibody (HL1929) - Azide and BSA Free [NBP3-25451] -

Western Blot: Enterovirus 71 VP1 Antibody (HL1929) - Azide and BSA Free [NBP3-25451] - Non-infected (-) and infected (+) RD whole cell extracts (5 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [HL1929] (NBP3-25451) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Enterovirus 71 VP1 Antibody (HL1929) - Azide and BSA Free

Western Blot: Enterovirus 71 VP1 Antibody (HL1929) [NBP3-25451] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [HL1929] (NBP3-25451) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Enterovirus 71 VP1 Antibody (HL1929) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Enterovirus 71 VP1

Alternate Names

Capsid protein VP1, EV71 VP1, P1D, Virion protein 1

Additional Enterovirus 71 VP1 Products

Product Documents for Enterovirus 71 VP1 Antibody (HL1929) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Enterovirus 71 VP1 Antibody (HL1929) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Enterovirus 71 VP1 Antibody (HL1929) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review Enterovirus 71 VP1 Antibody (HL1929) - Azide and BSA Free and earn rewards!

Have you used Enterovirus 71 VP1 Antibody (HL1929) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies