Equine IFN-gamma Antibody [Biotin]

Name: Equine IFN-gamma Antibody [Biotin]
Brand: R&D Systems
SKU: BAF1586
Price: 489 USD
Availability: InStock

R&D Systems | Catalog # BAF1586

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Equine

Applications

Western Blot, ELISA Detection (Matched Antibody Pair)

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all IFN-gamma Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant equine IFN-gamma
Ala25-Gln166
Accession # P42160

Specificity

Detects equine IFN-gamma in ELISAs and Western blots. In sandwich immunoassays, less than 20% cross-reactivity with recombinant canine IFN‑ gamma is observed, less than 6% cross-reactivity with recombinant bovine IFN-gamma and recombinant feline IFN-gamma is observed, and less than 0.2% cross‑reactivity with recombinant human IFN-gamma, recombinant mouse IFN-gamma, recombinant rat IFN-gamma, recombinant porcine IFN‑ gamma, recombinant rhesus macaque IFN-gamma, and recombinant cotton rat IFN-gamma is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Equine IFN-gamma Antibody [Biotin]

Equine IFN-gamma ELISA Standard Curve

Recombinant Equine IFN‑ gamma (Catalog # 1586-HG) was serially diluted and captured by Goat Anti-Equine IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1586) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Equine IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1586) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Equine IFN-gamma Antibody [Biotin]

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Equine IFN-gamma (Catalog # 1586-HG)

Equine IFN-gamma Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:

Capture Reagent: Equine IFN‑ gamma Antibody (Catalog # AF1586)
Standard: Recombinant Equine IFN-gamma Protein (Catalog # 1586-HG)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IFN-gamma

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature equine IFN-gamma exists as a noncovalently linked homodimer of 20‑25 kDa variably glycosylated subunits (3, 4). It shares 73%‑82% amino acid sequence identity with bovine, canine, feline, and porcine IFN-gamma and 42%‑64% with cotton rat, human, mouse, rat, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (5, 6). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (7). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (7, 8). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (9, 10). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (8).