EVI-1 Antibody

Novus Biologicals | Catalog # NBP1-68842

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all EVI-1 Primary Antibodies »

Product Specifications

Immunogen

EVI1 / AML1, internal region

Epitope

C-DKESLHSTSH

Reactivity Notes

Human

Specificity

This antibody is expected to recognize all reported isoforms (NP_001098547.3; NP_005232.2; NP_004982.2; NP_001157471.1; NP_001157472.1). Reported variants represent identical protein: NP_001098548.2, NP_005232.2

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for EVI-1 Antibody

EVI-1 Antibody

Western Blot: Goat Polyclonal EVI-1 Antibody [NBP1-68842] -

Western Blot: Goat Polyclonal EVI-1 Antibody [NBP1-68842] - (3µg/ml) staining of A431 (A), Caco-2 (B) and HeLa (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Applications for EVI-1 Antibody

Application
Recommended Usage

ELISA

1:16000

Western Blot

1-3 ug/ml
Application Notes
Approx. 140-150kDa band observed in lysates of cell lines A431, Caco-2 and HeLa (calculated MW of 126kDa according to NP_001098547.3). This molecular weight is routinely observed by other commercial sources.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EVI-1

EVI1 promotes cell proliferation by interacting with BRG1 and blocking the repression of BRG1 on E2F1 activity.

Long Name

Ecotropic Viral Integration Site 1

Alternate Names

EVI1, MDS1, MECOM, PRDM3

Entrez Gene IDs

2122 (Human)

Gene Symbol

MECOM

UniProt

NP_001098547.3 (Human)

Additional EVI-1 Products

Product Documents for EVI-1 Antibody

Certificate of Analysis

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Product Specific Notices for EVI-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Hematopoietic Stem Cell Markers

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