Exendin-4 Antibody (35) - BSA Free

Novus Biologicals | Catalog # NBP1-05179

Novus Biologicals
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Key Product Details

Species Reactivity

Reptile

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 35

Format

BSA Free
View all Exendin-4 Primary Antibodies »

Product Specifications

Immunogen

Synthetic exendin-4

Epitope

The epitope is in the 9-39 region of the peptide, and the antibody cross-reacts strongly with exedin (9-39)amide

Reactivity Notes

Reacts with Gila monster Heloderma suspectum

Specificity

Specific for exendin-4. No cross-reactivity with GLP-1, GLP-2 (human) or glucagon coated on ELISA wells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Concentration for any given batch will be between 0.85 - 1.15 mg/mL.

Scientific Data Images for Exendin-4 Antibody (35) - BSA Free

ELISA: Exendin-4 Antibody (35) - BSA Free [NBP1-05179]

ELISA: Exendin-4 Antibody (35) - BSA Free [NBP1-05179]

ELISA: Exendin-4 Antibody (35) - BSA Free [NBP1-05179] - Analysis using Biotin conjugate of NBP1-05179. NBP1-05180 is used as capture antibody in combination with a biotinylated detection antibody from the NBP2-23578 series. The best sensitivity in an unoptimized buffer assay was obtained with biotinylated NBP1-05179 as the detection antibody.

Applications for Exendin-4 Antibody (35) - BSA Free

Application
Recommended Usage

ELISA

1:2000
Application Notes
ELISA: NBP1-05179 binds to exendin-4 when coated on ELISA wells and reacts specifically with exendin-4 in solution giving a Ka of 1.1x10E9 in an inhibition ELISA. The binding between NBP1-05179 and exendin-4 is disrupted by 4.5 M MgCl2. NBP1-05179 (as biotinylated detection antibody) forms a sandwich ELISA pair with NBP1-05180 (as capture antibody) for measuring exendin-4, giving a detection limit of as little as 40 pmol/L in an unoptimized buffer assay. (1)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Exendin-4

Exendin-4 is a 39-amino-acid peptide found in venom from the Gila monster Helicoderma suspectum (1). It is a member of the glucagon-secretin family of peptide hormones and neuropeptides. Exendin-4 is a potent agonist of the GLP-1 receptor and hence a potent stimulator of insulin secretion (2).

Alternate Names

Exendin4

Additional Exendin-4 Products

Product Documents for Exendin-4 Antibody (35) - BSA Free

Certificate of Analysis

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Product Specific Notices for Exendin-4 Antibody (35) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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