FAK (Focal Adhesion Kinase) is a non-receptor protein tyrosine kinase involved in signal transduction from integrin-enriched focal adhesion sites that mediate cell contact with the extracellular matrix. FAK-enhanced signals have been shown to mediate the survival of anchorage-dependent cells and are critical for efficient cell migration in response to growth factor receptor and integrin stimulation. Elevated expression of FAK in human tumors has been correlated with increased malignancy and invasiveness.
FAK [p Tyr576] Antibody (HL127) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-13675
Recombinant Monoclonal Antibody
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Validated by
Knockout/Knockdown, Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL127 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr576 of human FAK. The exact sequence is proprietary.
Modification
p Tyr576
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for FAK [p Tyr576] Antibody (HL127) - Azide and BSA Free
Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] - HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody [HL127] (NBP3-13675) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody [HL127] (NBP3-13675) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
![FAK [p Tyr576] Antibody (HL127)](https://resources.rndsystems.com/images/products/nbp3-13675_rabbit-fak-p-tyr-576-mab-hl127-910202420131253.jpg "Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] -")
Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] -
Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK antibody [HL127] (NBP3-13675) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.![FAK [p Tyr576] Antibody (HL127)](https://resources.rndsystems.com/images/products/nbp3-13675_rabbit-fak-p-tyr-576-mab-hl127-1610202419405780.jpg "Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] -")
Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] -
Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody [HL127] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.![FAK [p Tyr576] Antibody (HL127) - Azide and BSA Free](https://resources.rndsystems.com/images/products/nbp3-13675_rabbit-fak-p-tyr-576-mab-hl127-western-blot-2611202519361.jpg "Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] -")
Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] -
Untreated (-) and treated (+) PC-12 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody [HL127] (NBP3-13675) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for FAK [p Tyr576] Antibody (HL127) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FAK
Long Name
Focal adhesion kinase 1
Alternate Names
FADK1, PTK2
Gene Symbol
PTK2
Additional FAK Products
Product Documents for FAK [p Tyr576] Antibody (HL127) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FAK [p Tyr576] Antibody (HL127) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
