FGF-5 Antibody (1B4) - Azide and BSA Free

Novus Biologicals | Catalog # H00002250-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Sheep

Applications

Western Blot, ELISA, Sandwich ELISA, Immunoprecipitation, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1B4

Format

Azide and BSA Free
View all FGF-5 Primary Antibodies »

Product Specifications

Immunogen

FGF5 (NP_004455.2, 159 a.a. ~ 268 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DCKFRERFQENSYNTYASAIHRTEKTGREWYVALNKRGKAKRGCSPRVKPQHISTHFLPRFKQSEQPELSFTVTVPEKKKPPSPIKPKIPLSAPRKNTNSVKYRLKFRFG

Specificity

Reacts with fibroblast growth factor 5.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for FGF-5 Antibody (1B4) - Azide and BSA Free

Western Blot: FGF-5 Antibody (1B4) [H00002250-M01]

Western Blot: FGF-5 Antibody (1B4) [H00002250-M01]

Western Blot: FGF-5 Antibody (1B4) [H00002250-M01] - FGF5 monoclonal antibody (M01), clone 1B4. Analysis of FGF5 expression in human colon.
Proximity Ligation Assay: FGF-5 Antibody (1B4) [H00002250-M01]

Proximity Ligation Assay: FGF-5 Antibody (1B4) [H00002250-M01]

Proximity Ligation Assay: FGF-5 Antibody (1B4) [H00002250-M01] - Analysis of protein-protein interactions between FGFR2 and FGF5. HeLa cells were stained with anti-FGFR2 rabbit purified polyclonal 1:1200 and anti-FGF5 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunoprecipitation: FGF-5 Antibody (1B4) [H00002250-M01]

Immunoprecipitation: FGF-5 Antibody (1B4) [H00002250-M01]

Immunoprecipitation: FGF-5 Antibody (1B4) [H00002250-M01] - Analysis of FGF5 transfected lysate using anti-FGF5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FGF5 MaxPab rabbit polyclonal antibody.
Sandwich ELISA: FGF-5 Antibody (1B4) [H00002250-M01] - Detection limit for recombinant GST tagged FGF5 is 3 ng/ml as a capture antibody.

Applications for FGF-5 Antibody (1B4) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Proximity Ligation Assay

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FGF-5

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was identified as an oncogene, which confers transforming potential when transfected into mammalian cells. Targeted disruption of the homolog of this gene in mouse resulted in the phenotype of abnormally long hair, which suggested a function as an inhibitor of hair elongation. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Long Name

Fibroblast Growth Factor 5

Alternate Names

FGF5

Entrez Gene IDs

2250 (Human)

Gene Symbol

FGF5

UniProt

NP_004455 (Human)

Additional FGF-5 Products

Product Documents for FGF-5 Antibody (1B4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for FGF-5 Antibody (1B4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FGF-5 Antibody (1B4) - Azide and BSA Free

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