Flightless I Antibody

Western Blot: Flightless I Antibody [NBP3-32341]

Western Blot: Flightless I Antibody [NBP3-32341] - Western blot analysis of Flightless I on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32341, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse lung tissue lysate
Lane 2: MCF-7 cell lysate

Immunohistochemistry: Flightless I Antibody [NBP3-32341]

Immunohistochemistry: Flightless I Antibody [NBP3-32341] - Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Flightless I antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32341, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: Flightless I Antibody [NBP3-32341]

Western blot analysis on different lysates with NBP3-32341 at 1/10,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate Lane 3: A549 (Human lung adenocarcinoma cell) cell lysate Lane 4: MCF7 (Human breast cancer cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 20 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP3-32341, 1/10,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 145 kDa Observed band size: 145 kDa