FoxP1 Antibody (JC12) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80742
![Western Blot: FoxP1 Antibody (JC12)Azide and BSA Free [NBP2-80742]](https://resources.rndsystems.com/images/products/FoxP1-Antibody-JC12-Azide-and-BSA-Free-Western-Blot-NBP2-80742-img0001.jpg "Western Blot: FoxP1 Antibody (JC12)Azide and BSA Free [NBP2-80742]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # JC12
Format
Azide and BSA Free
Loading...
Product Specifications
Immunogen
Human FOXP1 [Uniprot# Q9H334]
Epitope
Within the C-terminus
Localization
Nuclear, Cytoplasmic
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for FoxP1 Antibody (JC12) - Azide and BSA Free
Western Blot: FoxP1 Antibody (JC12)Azide and BSA Free [NBP2-80742]
Western Blot: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742] - Western blot analysis of resonicated MCF7 cell lysate (A) and MCF7 cell lysate (B) using FOXP1 antibody at 2 ug/ml. Image from the standard format of this antibody.![Immunocytochemistry/ Immunofluorescence: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]](https://resources.rndsystems.com/images/products/FoxP1-Antibody-JC12-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80742-img0002.jpg "Immunocytochemistry/ Immunofluorescence: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]")
Immunocytochemistry/ Immunofluorescence: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]
Immunocytochemistry/Immunofluorescence: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742] - FOXP1 antibody was tested at 1:25 in MCF-7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x. Image from the standard format of this antibody.![Immunohistochemistry-Paraffin: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]](https://resources.rndsystems.com/images/products/FoxP1-Antibody-JC12-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80742-img0006.jpg "Immunohistochemistry-Paraffin: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]")
Immunohistochemistry-Paraffin: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]
Immunohistochemistry-Paraffin: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742] - IHC analysis of formalin-fixed paraffin-embedded tissue section of malignant stromal tumor of the human small bowel using mouse monoclonal FOXP1 antibody (clone JC12) at 5 ug/ml concentration. The carcinoma cells developed an expected and specific strong![Flow Cytometry: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]](https://resources.rndsystems.com/images/products/FoxP1-Antibody-JC12-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80742-img0007.jpg "Flow Cytometry: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]")
Flow Cytometry: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]
Flow Cytometry: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HeLa (B) cells using FOXP1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.![Immunohistochemistry: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]](https://resources.rndsystems.com/images/products/FoxP1-Antibody-JC12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80742-img0003.jpg "Immunohistochemistry: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]")
Immunohistochemistry: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]
Immunohistochemistry: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal brain using mouse monoclonal FOXP1 antibody (clone JC12) at 5 ug/ml concentration. The cells in the brain tissue depicted strong specific nuclear along with relatively weak cy![Immunohistochemistry-Paraffin: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]](https://resources.rndsystems.com/images/products/FoxP1-Antibody-JC12-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80742-img0004.jpg "Immunohistochemistry-Paraffin: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]")
Immunohistochemistry-Paraffin: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]
Immunohistochemistry-Paraffin: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal breast using FOXP1 antibody (clone JC12) at 5 ug/ml concentration. The breast ductal/acinar epithelial cells and the myoepithelial cells developed a strong nuclear along with![Immunohistochemistry-Paraffin: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]](https://resources.rndsystems.com/images/products/FoxP1-Antibody-JC12-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80742-img0005.jpg "Immunohistochemistry-Paraffin: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]")
Immunohistochemistry-Paraffin: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]
Immunohistochemistry-Paraffin: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal colon using mouse monoclonal FOXP1 antibody (clone JC12) at 5 ug/ml concentration. Most of the cells depicted an expected strong nuclear with mild cytoplasmic staining. ImageApplications for FoxP1 Antibody (JC12) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1 ug per million cells
Immunocytochemistry/ Immunofluorescence
reported in scientific literature (PMID 15161711)
Immunohistochemistry-Frozen
1:10-1:500
Immunoprecipitation
reported in scientific literature (PMID 17586580)
Western Blot
2ug/ml
Application Notes
Heat induced antigen retrieval with Sodium Citrate buffer pH 6.0 is recommended when using this antibody for IHC-P. This antibody is CyTOF ready.
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FoxP1
Long Name
Forkhead Box P1
Alternate Names
QRF1
Gene Symbol
FOXP1
Additional FoxP1 Products
Product Documents for FoxP1 Antibody (JC12) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FoxP1 Antibody (JC12) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for FoxP1 Antibody (JC12) - Azide and BSA Free
There are currently no reviews for this product. Be the first to review FoxP1 Antibody (JC12) - Azide and BSA Free and earn rewards!
Have you used FoxP1 Antibody (JC12) - Azide and BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...