FTO Antibody (AG103) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80051
![Western Blot: FTO Antibody (AG103)Azide and BSA Free [NBP2-80051]](https://resources.rndsystems.com/images/products/FTO-Antibody-AG103-Western-Blot-NBP2-80051-img0003.jpg "Western Blot: FTO Antibody (AG103)Azide and BSA Free [NBP2-80051]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # AG103
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant human FTO.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for FTO Antibody (AG103) - Azide and BSA Free
Western Blot: FTO Antibody (AG103)Azide and BSA Free [NBP2-80051]
Western Blot: FTO Antibody (AG103) [NBP2-80051] - Analysis of human FTO using anti-human FTO mAb (AG103) at 1:1,000 dilution:1. hFTO (His-tagged) 100ng.2. HEK293T 100ug.3. HEK293E 100ug.4. HepG2 100ug.5. Hep3B 50ug.6. VIT1 80ug.7. THP1 74ug.8. Molt4 96ug.9. A549 48ug![Immunohistochemistry-Paraffin: FTO Antibody (AG103) - Azide and BSA Free [NBP2-80051]](https://resources.rndsystems.com/images/products/FTO-Antibody-AG103-Immunohistochemistry-Paraffin-NBP2-80051-img0001.jpg "Immunohistochemistry-Paraffin: FTO Antibody (AG103) - Azide and BSA Free [NBP2-80051]")
Immunohistochemistry-Paraffin: FTO Antibody (AG103) - Azide and BSA Free [NBP2-80051]
Immunohistochemistry-Paraffin: FTO Antibody (AG103) [NBP2-80051] - Immunohistochemical staining of FTO with anti-FTO (human), mAb (AG103) in brain, hypothalamus and paraventricular nucleus (5~10 ug/mL). This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalinfixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain (40x).![Immunoprecipitation: FTO Antibody (AG103) - Azide and BSA Free [NBP2-80051]](https://resources.rndsystems.com/images/products/FTO-Antibody-AG103-Immunoprecipitation-NBP2-80051-img0002.jpg "Immunoprecipitation: FTO Antibody (AG103) - Azide and BSA Free [NBP2-80051]")
Immunoprecipitation: FTO Antibody (AG103) - Azide and BSA Free [NBP2-80051]
Immunoprecipitation: FTO Antibody (AG103) [NBP2-80051] - Immunoprecipitation of recombinant human FTO proteins using anti-FTO (human), mAb (AG103) at different concentrations. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by Western blot with rabbit anti-mouse FTO pAb.1. hFTO (His-tagged) recombinant control 100ng.2. hFTO (His-tagged) 5ug.3. hFTO (His-tagged) 2.5ug.4. hFTO (His-tagged) 1.25ug.5. hFTO (His-tagged) 0.625ug.6. hFTO (His-tagged) 0.312ug.7. hFTO (His-tagged) 0.156ug.8. hFTO (His-tagged) 0.078ugApplications for FTO Antibody (AG103) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:2000 - 1:5000
Immunohistochemistry
5 - 10 ug/mL
Immunohistochemistry-Paraffin
5 - 10 ug/mL
Immunoprecipitation
5 ug/mL
Western Blot
1:2000 - 1:5000
Application Notes
Immunoprecipitation: 5 ug/ml per sample in a final reaction volume of 500-1000 ul.
Western Blot: 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature.
Western Blot: 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
0.2 um-filtered solution in PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FTO
FTO antibodies are useful tools for lipid metabolism research and obesity studies.
Long Name
Fat Mass and Obesity Associated
Alternate Names
Alpha-ketoglutarate-dependent dioxygenase FTO
Gene Symbol
FTO
Additional FTO Products
Product Documents for FTO Antibody (AG103) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FTO Antibody (AG103) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for FTO Antibody (AG103) - Azide and BSA Free
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Q: Do you have any antibodies against FTO, where you can indicate the sequence of the immunogen?
A: With regards to other FTO antibodies that we do list the peptide sequence for, the immunogen sequence for catalog number NB110-60935 can be found in the range of amino acids 400-505. Many of the exact immunogen sequences used for our products are proprietary and cannot be disclosed. In these instances we try to provide a reasonable amino acid range to help in your decision.
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