G9a/EHMT2 Antibody (23GB820)
Novus Biologicals | Catalog # NBP3-52006
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB820
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Product Specifications
Immunogen
A synthesized peptide derived from human G9a/EHMT2 (Uniprot: Q96KQ7)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for G9a/EHMT2 Antibody (23GB820)
Flow Cytometry: G9a/EHMT2 Antibody (23GB820)[NBP3-52006] -
Validation of G9a/EHMT2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with G9a/EHMT2 antibody (1:2,000) and analyzed using CytoFLEX.
Flow Cytometry: G9a/EHMT2 Antibody (23GB820)[NBP3-52006] -
Flow cytometric analysis of G9a/EHMT2expression in HeLa cells using G9a/EHMT2 antibody (1:2,000). Green, isotype control; red, EHMT2/G9A.
Western Blot: G9a/EHMT2 Antibody (23GB820)[NBP3-52006] -
Western blotting analysis using G9a/EHMT2 antibody (1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.Applications for G9a/EHMT2 Antibody (23GB820)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: G9a/EHMT2
Long Name
G9a Histone Methyltransferase/Euchromatic Histone-lysine N-methyltransferase 2
Alternate Names
EHMT2
Gene Symbol
EHMT2
Additional G9a/EHMT2 Products
Product Documents for G9a/EHMT2 Antibody (23GB820)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for G9a/EHMT2 Antibody (23GB820)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Cellular Response to Hypoxia Protocols
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
