G9a/EHMT2 Antibody - BSA Free
Novus Biologicals | Catalog # NB100-40825
![Western Blot: G9a/EHMT2 Antibody [NB100-40825]](https://resources.rndsystems.com/images/products/G9a-EHMT2-Antibody-Western-Blot-NB100-40825-img0006.jpg "Western Blot: G9a/EHMT2 Antibody [NB100-40825]")
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Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1160 and the C-terminus (residue 1210) of human G9A (Euchromatic Histone-Lysine N-methyltransferase 2) using the numbering given in entry NP_006700.2 (GeneID 10919).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for G9a/EHMT2 Antibody - BSA Free
Western Blot: G9a/EHMT2 Antibody [NB100-40825]
Western Blot: G9a/EHMT2 Antibody [NB100-40825] - Detection of Human G9A/EHMT2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G9A/EHMT2 antibody NB100-40825 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunohistochemistry-Paraffin: G9a/EHMT2 Antibody [NB100-40825]](https://resources.rndsystems.com/images/products/G9a-EHMT2-Antibody-Immunohistochemistry-NB100-40825-img0007.jpg "Immunohistochemistry-Paraffin: G9a/EHMT2 Antibody [NB100-40825]")
Immunohistochemistry-Paraffin: G9a/EHMT2 Antibody [NB100-40825]
Immunohistochemistry-Paraffin: G9a/EHMT2 Antibody [NB100-40825] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-G9A/EHMT2 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB![Immunoprecipitation: G9a/EHMT2 Antibody [NB100-40825]](https://resources.rndsystems.com/images/products/G9a-EHMT2-Antibody-Immunoprecipitation-NB100-40825-img0008.jpg "Immunoprecipitation: G9a/EHMT2 Antibody [NB100-40825]")
Immunoprecipitation: G9a/EHMT2 Antibody [NB100-40825]
Immunoprecipitation: G9a/EHMT2 Antibody [NB100-40825] - Detection of human G9A/EHMT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G9A/EHMT2 antibody NB100-40825 (lot NB100-40825-2) used for IP at 6 ug per reaction. G9A/EHMT2 was also immunoprecipitated by a previous lot of this antibody (NB100-40825-1) and another rabbit anti-G9A/EHMT2 antibody. For blotting immunoprecipitated G9A/EHMT2, NB100-40825 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for G9a/EHMT2 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:1000 -1:5000
Immunoprecipitation
2-10 ug/mg lysate
Application Notes
While trace amounts of G9A/EHMT2 can be immunoprecipitated by this antibody, the efficiency is very low. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Reviewed Applications
Read 1 review rated 3 using NB100-40825 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: G9a/EHMT2
Long Name
G9a Histone Methyltransferase/Euchromatic Histone-lysine N-methyltransferase 2
Alternate Names
EHMT2
Entrez Gene IDs
10919 (Human)
Gene Symbol
EHMT2
UniProt
Additional G9a/EHMT2 Products
Product Documents for G9a/EHMT2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for G9a/EHMT2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for G9a/EHMT2 Antibody - BSA Free
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1 Customer Rating
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Application: Western BlotSample Tested: 293T Cell Lysate, Sample Amount: 50 ugSpecies: HumanVerified Customer | Posted 01/26/2010
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
