GAD1/GAD67 Antibody (CL2919) - BSA Free

Novus Biologicals | Catalog # NBP2-46639

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL2919

Format

BSA Free
View all GAD1/GAD67 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q99259, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for GAD1/GAD67 Antibody (CL2919) - BSA Free

Western Blot: GAD1/GAD67 Antibody (CL2919) [NBP2-46639]

Western Blot: GAD1/GAD67 Antibody (CL2919) [NBP2-46639]

Western Blot: GAD1/GAD67 Antibody (2919) [NBP2-46639] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue
Immunohistochemistry: GAD1/GAD67 Antibody (CL2919) [NBP2-46639]

Immunohistochemistry: GAD1/GAD67 Antibody (CL2919) [NBP2-46639]

Immunohistochemistry: GAD1/GAD67 Antibody (2919) [NBP2-46639] - Staining of human tonsil shows absence of immunoreactivity (negative control).
Western Blot: GAD1/GAD67 Antibody (CL2919) [NBP2-46639]

Western Blot: GAD1/GAD67 Antibody (CL2919) [NBP2-46639]

Western Blot: GAD1/GAD67 Antibody (2919) [NBP2-46639] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue
Immunohistochemistry: GAD1/GAD67 Antibody (CL2919) [NBP2-46639]

Immunohistochemistry: GAD1/GAD67 Antibody (CL2919) [NBP2-46639]

Immunohistochemistry: GAD1/GAD67 Antibody (2919) [NBP2-46639] - Staining of rat hippocampal formation shows strong positivity in GABAergic fibers in the CA1 area.
Immunohistochemistry: GAD1/GAD67 Antibody (CL2919) [NBP2-46639]

Immunohistochemistry: GAD1/GAD67 Antibody (CL2919) [NBP2-46639]

Immunohistochemistry: GAD1/GAD67 Antibody (2919) [NBP2-46639] - Staining of mouse cerebral cortex shows strong immunoreactivity in GABAergic synapses on pyramidal neurons.
Immunohistochemistry: GAD1/GAD67 Antibody (CL2919) [NBP2-46639]

Immunohistochemistry: GAD1/GAD67 Antibody (CL2919) [NBP2-46639]

Immunohistochemistry: GAD1/GAD67 Antibody (2919) [NBP2-46639] - Staining of human cerebral cortex shows immunoreactivity in neuropil and in some neuronal cells.
Immunohistochemistry: GAD1/GAD67 Antibody (CL2919) [NBP2-46639]

Immunohistochemistry: GAD1/GAD67 Antibody (CL2919) [NBP2-46639]

Immunohistochemistry: GAD1/GAD67 Antibody (2919) [NBP2-46639] - Staining of rat substantia nigra shows strong immunoreactivity in GABAergic fibers.
Immunohistochemistry: GAD1/GAD67 Antibody (CL2919) [NBP2-46639]

Immunohistochemistry: GAD1/GAD67 Antibody (CL2919) [NBP2-46639]

Immunohistochemistry: GAD1/GAD67 Antibody (2919) [NBP2-46639] - Staining of mouse globus pallidus shows strong positivity in GABAergic fibers.
Immunohistochemistry: GAD1/GAD67 Antibody (CL2919) [NBP2-46639]

Immunohistochemistry: GAD1/GAD67 Antibody (CL2919) [NBP2-46639]

Immunohistochemistry: GAD1/GAD67 Antibody (2919) [NBP2-46639] - Staining of human cerebellum shows strong immunoreactivity in Purkinje cells, as well as in fibers in granular and molecular layers.
Immunohistochemistry: GAD1/GAD67 Antibody (CL2919) [NBP2-46639]

Immunohistochemistry: GAD1/GAD67 Antibody (CL2919) [NBP2-46639]

Immunohistochemistry: GAD1/GAD67 Antibody (2919) [NBP2-46639] - Staining of rat globus pallidus shows strong positivity in GABAergic fibers.
Immunohistochemistry: GAD1/GAD67 Antibody (CL2919) [NBP2-46639]

Immunohistochemistry: GAD1/GAD67 Antibody (CL2919) [NBP2-46639]

Immunohistochemistry: GAD1/GAD67 Antibody (2919) [NBP2-46639] - Staining of mouse cerebellum shows moderate immunoreactivity in Purkinje cells, as well as in fibers in granular and molecular layers.

Applications for GAD1/GAD67 Antibody (CL2919) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAD1/GAD67

Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA). There are two known forms of GAD; GAD65 (65 kDa) and GAD67 (67 kDa) that are encoded by two different genes and appear to have distinct intraneuronal distribution in the brain. This antibody reacts with GAD67 and is predicted to react with GAD65.

Long Name

Glutamate Decarboxylase 1 (Brain, 67kDa)

Alternate Names

GAD67

Entrez Gene IDs

2571 (Human)

Gene Symbol

GAD1

UniProt

Q99259

Additional GAD1/GAD67 Products

Product Documents for GAD1/GAD67 Antibody (CL2919) - BSA Free

Certificate of Analysis

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Product Specific Notices for GAD1/GAD67 Antibody (CL2919) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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