GAD2/GAD65 Antibody (CL2736) - BSA Free

Novus Biologicals | Catalog # NBP2-46629

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2736

Format

BSA Free
View all GAD2/GAD65 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q05329, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for GAD2/GAD65 Antibody (CL2736) - BSA Free

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Analysis in human cerebral cortex and tonsil tissues using NBP2-46629 antibody. Corresponding GAD2/GAD65 RNA-seq data are presented for the same tissues.
Western Blot: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Western Blot: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Western Blot: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue
Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of mouse midbrain shows moderate positivity in GABergic neural fibers in the substantia nigra.
Western Blot: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Western Blot: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Western Blot: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue
Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of rat basal forebrain shows moderate immunoreactivity in GABAergic fibers.
Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of mouse substantia nigra shows moderate positivity in GABAergic fibers in pars reticulata.
Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of rat globus pallidus shows moderate positivity in GABAergic neural fibers.
Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of human cerebellum shows moderate immunoreactivity in GABAergic fibers in granular and molecular layers.
Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of human cerebral cortex shows moderate to strong positivity in GABAergic neuronal processes.
Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of human pancreas shows moderate cytoplasmic positivity in endocrine glandular cells.
Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629]

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.

Applications for GAD2/GAD65 Antibody (CL2736) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAD2/GAD65

Antibodies to glutamic acid decarboxylase-65 (GAD65) are present in autoimmune disorders such as insulin-dependent (type 1) diabetes mellitus (IDDM), stiff man syndrome and polyendocrine autoimmune disease. Auto-antibodies to GAD65 are present in 60-70% of individuals with newly diagnosed IDDM and thus are important markers for disease activity. These auto-antibodies usually recognize conformation-dependent regions on GAD65 and rarely bind to the second isoform, GAD67. Auto-antibodies to GAD67 are found in only 15% of recent-onset IDDM patients and most of this binding can be blocked with GAD65, suggesting shared epitopes between the two isoforms of GAD.

Long Name

Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa)

Alternate Names

GAD65

Entrez Gene IDs

2572 (Human)

Gene Symbol

GAD2

UniProt

Q05329

Additional GAD2/GAD65 Products

Product Documents for GAD2/GAD65 Antibody (CL2736) - BSA Free

Certificate of Analysis

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Product Specific Notices for GAD2/GAD65 Antibody (CL2736) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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