GCFC1 Antibody

Novus Biologicals | Catalog # NBP1-71921

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all GCFC1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 225 and 275 of human GC-rich Sequence DNA-binding Factor 1 using the numbering given in entry NP_057715.2 (GeneID 94104).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GCFC1 Antibody

Western Blot: GCFC1 Antibody [NBP1-71921]

Western Blot: GCFC1 Antibody [NBP1-71921]

Western Blot: GCFC1 Antibody [NBP1-71921] - Detection of Human GCFC1 by Western Blot. Samples: Whole cell lysate ( 15 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GCFC1 antibody NBP1-71921 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: GCFC1 Antibody [NBP1-71921]

Western Blot: GCFC1 Antibody [NBP1-71921]

Western Blot: GCFC1 Antibody [NBP1-71921] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-GCFC1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. GCFC1 was also immunoprecipitated by rabbit anti-GCFC1 antibodies NBP1-71922 and NBP1-71923, which recognize downstream epitopes.

Applications for GCFC1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 mcg/mg

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GCFC1

Similarity to a transcriptional repressor suggests that this gene's protein product is involved in the regulation of transcription. Alternative splicing of this gene results in three transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined. [provided by RefSeq]

Alternate Names

BM020, C21orf66, chromosome 21 open reading frame 66, FLJ90561, fSAP105, functional spliceosome-associated protein 105, GCFCFSAP105, GC-rich sequence DNA-binding factor 1, GC-rich sequence DNA-binding factor candidate

Gene Symbol

PAXBP1

UniProt

NP_057715.2 (Human)

Additional GCFC1 Products

Product Documents for GCFC1 Antibody

Certificate of Analysis

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Product Specific Notices for GCFC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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