GCLM Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33405

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GCLM Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GCLM. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GCLM Antibody - BSA Free

Knockout Validated: GCLM Antibody [NBP1-33405]

Western Blot: GCLM Antibody [NBP1-33405]

Western Blot: GCLM Antibody [NBP1-33405] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody [N1N3]. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: GCLM Antibody [NBP1-33405]

Western Blot: GCLM Antibody [NBP1-33405]

Western Blot: GCLM Antibody [NBP1-33405] - Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE; antibody diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: GCLM Antibody [NBP1-33405]

Immunocytochemistry/ Immunofluorescence: GCLM Antibody [NBP1-33405]

Immunocytochemistry/Immunofluorescence: GCLM Antibody [NBP1-33405] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: GCLM Antibody [NBP1-33405]

Immunohistochemistry-Paraffin: GCLM Antibody [NBP1-33405]

Immunohistochemistry-Paraffin: GCLM Antibody [NBP1-33405] - BT474 xenograft, using GCLM antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for GCLM Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000

Reviewed Applications

Read 2 reviews rated 4 using NBP1-33405 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GCLM

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.

Alternate Names

Gamma-ECS regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, GCS light chain, GLCLRglutamate--cysteine ligase regulatory subunit, glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory(30.8kD), Glutamate--cysteine ligase modifier subunit, glutamate-cysteine ligase regulatory protein, glutamate-cysteine ligase, modifier subunit, GSC light chain

Gene Symbol

GCLM

Additional GCLM Products

Product Documents for GCLM Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GCLM Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for GCLM Antibody - BSA Free

Customer Reviews for GCLM Antibody - BSA Free (2)

4 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
0%
3 Stars
50%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • GCLM Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human Cancer Cells
    Species: Human
    Verified Customer | Posted 09/18/2018
    This antibody was used to detect GCLM expression in control (NTC) and different GCLM knockdown subclones in human breast cancer cell line by western blot.
    GCLM Antibody - BSA Free NBP1-33405
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Human cancer cell whole cell lysate
    Species: Human
    Verified Customer | Posted 07/31/2015
    GCLM expression in breast cancer cell lines. NBP1-33045
    GCLM Antibody - BSA Free NBP1-33405

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Protocols

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FAQs

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