GDF-15 Antibody (6D12.H10.E4) - C-terminus - BSA Free

Novus Biologicals | Catalog # NBP2-44214

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Lambda Clone # 6D12.H10.E4

Format

BSA Free
View all GDF-15 Primary Antibodies »

Product Specifications

Immunogen

This Protein-A purified GDF-15 Antibody (6D12.H10.E4) was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E.coli corresponding to C-term mouse NAG-1 protein. Cross reactivity to MBP is negative. (Uniprot: Q99988)

Reactivity Notes

A BLAST analysis suggests reactivity with GDF-15 from chimpanzee and macaque based on a 100% homology. Partial reactivity is expected against rat based on an 86% homology with the immunizing sequence. Cross-reactivity with GDF-15 from other sources has not been determined.

Specificity

This antibody reacts with the C-terminus of endogenous GDF-15 protein from mouse tissues. A BLAST analysis suggests reactivity with GDF-15 from chimpanzee and macaque based on a 100% homology. Partial reactivity is expected against rat based on an 86% homology with the immunizing sequence. Cross-reactivity with GDF-15 from other sources has not been determined.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Lambda

Description

This product was purified from concentrated tissue culture supernatant Protein G chromatography

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for GDF-15 Antibody (6D12.H10.E4) - C-terminus - BSA Free

Knockdown Validated: GDF-15 Antibody (6D12.H10.E4) - C-terminus [NBP2-44214]

Western Blot: GDF-15 Antibody (6D12.H10.E4) - C-terminus [NBP2-44214]

Western Blot: GDF-15 Antibody (6D12.H10.E4) [NBP2-44214] - Western blot shows lysates of HepG2 human liver carcinoma parental cell line and GDF-15 knockout (KO) HepG2 cell line. PVDF membrane was probed with 1:250 of Rat Anti-Human GDF-15 Polyclonal Antibody (Catalog # NBP2-44214) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog #HAF006). Specific band was detected for GDF-15 at approximately 33 kDa (as indicated) in the parental HepG2 cell line, but is not detectable in the knockout HepG2 cell line. This experiment was conducted under reducing conditions.
Western Blot: GDF-15 Antibody (6D12.H10.E4)C-terminus [NBP2-44214]

Western Blot: GDF-15 Antibody (6D12.H10.E4)C-terminus [NBP2-44214]

Western Blot: GDF-15 Antibody (6D12.H10.E4) [NBP2-44214] - Analysis of Rat Anti-Nag 1 (C terminal specific). Lane 1: Protein Standard Opal Pre-stained. Lane 2: MBP-NAG-1 fusion protein 1. Lane 3: MBP-NAG-1 fusion protein 2. Lane 4: MBP. Load 40ng. Primary Antibody: Rat anti-Nag 1 (C terminal specific). Secondary Antibody: Rat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed.
GDF-15 Antibody (6D12.H10.E4) - C-terminus

GDF-15 Antibody (6D12.H10.E4) - C-terminus

Western Blot of GDF-15 Antibody (6D12.H10.E4) - C-terminus. Lane 1: Opal Prestained Molecular Weight Marker

Applications for GDF-15 Antibody (6D12.H10.E4) - C-terminus - BSA Free

Application
Recommended Usage

ELISA

1:2000

Western Blot

1:1000
Application Notes
This Protein G This product has been tested by ELISA, SDS-PAGE, and western blot for mouse NAG-1 protein. Specific conditions for reactivity should be optimized by the end user. Expect bands in Western blots of native protein of approximately ~58kDa using the appropriate cell lysate or extract.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: GDF-15

Growth differentiation factor 15 (GDF15) is a transforming growth factor beta superfamily member that acts to regulate inflammatory and apoptotic responses in injured or diseased tissues. GDF15 is most strongly expressed in the liver, placenta and kidney.

GDF15 antibodies are useful for studies on inflamation and immune system research.

Long Name

Growth Differentiation Factor 15

Alternate Names

GDF15, MIC-1, NAG-1, PDF, PLAB, PTGF-beta

Gene Symbol

GDF15

UniProt

Q99988

Additional GDF-15 Products

Product Documents for GDF-15 Antibody (6D12.H10.E4) - C-terminus - BSA Free

Certificate of Analysis

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Product Specific Notices for GDF-15 Antibody (6D12.H10.E4) - C-terminus - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GDF-15 Antibody (6D12.H10.E4) - C-terminus - BSA Free

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