Glut1 Antibody

Novus Biologicals | Catalog # NB300-666

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Cited:

Human, Mouse, Rat, Fish, Fish - Danio rerio (Zebrafish)

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Glut1 Primary Antibodies »

Product Specifications

Immunogen

This Glut1 antibody is made against a synthetic peptide conjugated to KLH, corresponding to amino acids 478-492 of Human Glucose Transporter GLUT1. Peptide was covalently modified. Sequence: PEELFHPLGADSQV

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 26657775).

Localization

Integral membrane protein. Localizes primarily at the cell surface (by similarity).

Marker

Plasma Membrane Marker

Specificity

Glucose Transporter GLUT1

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glut1 Antibody

Western Blot: Glut1 Antibody [NB300-666]

Western Blot: Glut1 Antibody [NB300-666]

Western Blot: Glut1 Antibody [NB300-666] - Western blot analysis of 786-mock cells stably expressing shRNA constructs. For HIF2A (NB100-480) and HIF1B (NB100-124) analysis, nuclear extracts were generated and analyzed. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0023936) licensed under a CC-BY license. Glut-1 was detected using NB300-666 in whole cell extracts.
Western Blot: Glut1 Antibody [NB300-666]

Western Blot: Glut1 Antibody [NB300-666]

Western Blot: Glut1 Antibody [NB300-666] - WB analysis of purified Glucose Transporter (GLUT1) with GLUT1 antibody at a dilution of 1:2000.
Western Blot: Glut1 Antibody [NB300-666]

Western Blot: Glut1 Antibody [NB300-666]

Glut1-Antibody-Western-Blot-NB300-666-img0008.jpg
Western Blot: Glut1 Antibody [NB300-666]

Western Blot: Glut1 Antibody [NB300-666]

Western Blot: Glut1 Antibody [NB300-666] - Rat brain cortical extracts (10 ug total protein per lane) on 10% SDS-PAGE gel. Antibody diluted 1:2000.

Applications for Glut1 Antibody

Application
Recommended Usage

ELISA

1:12000

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Immunoprecipitation

1:200

Western Blot

1:5000
Application Notes
Immunoprecipitation: 2 uL will immunoprecipitate 80-85% Glut1 from rat hippocampal membranes. By Western Blot, a 50 kDa band is seen (predicted MW is 54.1 kDa). Optimal dilutions should be determined by the end user.

Reviewed Applications

Read 2 reviews rated 3.5 using NB300-666 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris/Glycine buffer (pH 7.4 - 7.8), HEPES, 0.5% BSA, 30% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glut1

Glucose transporter 1 (GLUT1) or solute carrier family 2 (SLC2A1) is a member of the GLUT family of monosaccharides and polyols transporters. GLUT proteins transport glucose across cellular membranes through facilitative mechanisms and play a key role in glucose homeostasis (1). Fourteen GLUT proteins have been identified in the human, which are encoded by SLC2A genes 1-14 and are broadly expressed in many cell types and tissues. GLUT family members differ in sequence homology, substrate specificity and expression patterns. Based on sequence homology, GLUT family members are classified into Class I (GLUT1, 2, 3, 4, and GLUT14), Class II (GLUT5, 7, 9, and 11), and Class III (GLUT6, 8, 10, 12 and 13) (1). Structurally, GLUT transporters are integral membrane glycoproteins consisting of 12 membrane spanning helical domains, a single N-linked glycosylation site, and having cytoplasmic facing carboxy and amino terminal domains (2).

GLUT1 (Human glycosylated form theoretical molecular weight 55kDa) functions primarily as a glucose transporter but can transport other substrates including mannose, galactose and glucosamine across the membrane (3). Like other GLUT family members, GLUT1 is broadly expressed, nevertheless it is the predominant glucose transporter expressed in red blood cells and brain endothelial cells (1). SLC2A1 mutations underscore the autosomal dominant disorder GLUT1 deficiency syndrome (GLUTI-DS) which is characterized by low glucose levels in the brain or hypoglycorrhachia due to insufficient glucose transport across the blood brain barrier (2, 4, 5). Phenotypically, GLUT1-DS is characterized by early onset seizures, neurologic developmental delay, microcephaly, and ataxia (4). GLUT1 is highly expressed in the endothelium of cutaneous vascular lesions and serves as a marker for the diagnosis of juvenile or infantile hemangiomas (6).

References

1. Augustin, R. (2010). The protein family of glucose transport facilitators: It's not only about glucose after all. IUBMB Life. https://doi.org/10.1002/iub.315

2. Mueckler, M., & Thorens, B. (2013). The SLC2 (GLUT) family of membrane transporters. Molecular Aspects of Medicine. https://doi.org/10.1016/j.mam.2012.07.001

3. Stein, W. D., & Litman, T. (2015). Carrier-Mediated Transport. In Channels, Carriers, and Pumps. https://doi.org/10.1016/b978-0-12-416579-3.00004-6

4. Pearson, T. S., Akman, C., Hinton, V. J., Engelstad, K., & De Vivo, D. C. (2013). Phenotypic spectrum of glucose transporter type 1 deficiency syndrome (Glut1 DS). Current Neurology and Neuroscience Reports. https://doi.org/10.1007/s11910-013-0342-7

5. Messana, T., Russo, A., Vergaro, R., Boni, A., Santucci, M., & Pini, A. (2018). Glucose transporter type 1 deficiency syndrome: Developmental delay and early-onset ataxia in a novel mutation of the SLC2A1 gene. Journal of Pediatric Neurosciences. https://doi.org/10.4103/JPN.JPN_169_17

6. van Vugt, L. J., van der Vleuten, C. J. M., Flucke, U., & Blokx, W. A. M. (2017). The utility of GLUT1 as a diagnostic marker in cutaneous vascular anomalies: A review of literature and recommendations for daily practice. Pathology Research and Practice. https://doi.org/10.1016/j.prp.2017.04.023

Long Name

Glucose Transporter Type 1

Alternate Names

DYT17, DYT18, DYT9, EIG12, GLUT1DS, SLC2A1

Entrez Gene IDs

6513 (Human); 20537 (Mouse); 25552 (Rat)

Gene Symbol

SLC2A1

UniProt

P11166 (Human)

Additional Glut1 Products

Product Documents for Glut1 Antibody

Certificate of Analysis

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Product Specific Notices for Glut1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Glut1 Antibody (2)

3.5 out of 5
2 Customer Ratings
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Customer Images

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Human cancer cell whole cell lysate
    Species: Human
    Verified Customer | Posted 10/04/2015
    Glut1 expression in MDA-MB-231 cells under normoxia and hypoxia.
    Glut1 Antibody NB300-666
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 24260413
    Species: Human
    Verified Customer | Posted 12/23/2014

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