Glycogen Synthase [p Ser641] Antibody (SR46-06)

Novus Biologicals | Catalog # NBP2-67315

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR46-06 expressed in HEK293
View all Glycogen Synthase Primary Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser641 of Human Glycogen Synthase. (SwissProt: P13807 Human; SwissProt: Q9Z1E4 Mouse; Entrez Gene: 690987 Rat)

Modification

p Ser641

Localization

Cytoplasm

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glycogen Synthase [p Ser641] Antibody (SR46-06)

Immunocytochemistry/Immunofluorescence: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Staining Phospho-Glycogen synthase 1(S641) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-Glycogen synthase [p Ser641] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunocytochemistry/Immunofluorescence: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Staining Phospho-Glycogen synthase 1(S641) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of paraffin-embedded mouse liver tissue using anti-Phospho-Glycogen synthase 1(S641) antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Phospho-Glycogen synthase 1(S641) antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of paraffin-embedded mouse smooth muscle tissue using anti-Phospho-Glycogen synthase 1(S641) antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-Phospho-Glycogen synthase 1(S641) antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Glycogen Synthase [p Ser641] Antibody (SR46-06) Western Blot: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315]

Western Blot: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315]

Western blot analysis of Phospho-Glycogen synthase 1 (S641) on HepG2 cell lysates. Lane 1: HepG2 cells, whole cell lysate, 10ug/lane Lane 2: HepG2 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-Glycogen synthase 1 (S641) antibody (NBP2-67315) at 1/500 dilution. Anti-GAPDH antibody at 1/10,000 dilution. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/200,000 dilution. Predicted band size: 84 kDa Observed band size: 84 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 1 minute 34 seconds
Glycogen Synthase [p Ser641] Antibody (SR46-06) Western Blot: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315]

Western Blot: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315]

Western blot analysis on different lysates with NBP2-67315 at 1/1,000 dilution. Lane 1: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 17 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP2-67315, 1/1,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 83.8 kDa Observed band size: 83.8 kDa
Glycogen Synthase [p Ser641] Antibody (SR46-06) Western Blot: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315]

Western Blot: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315]

Western blot analysis on different lysates with NBP2-67315 at 1/1,000 dilution. Lane 1: SK-BR-3 (Human breast cancer cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 17 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP2-67315, 1/1,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 83.8 kDa Observed band size: 83.8 kDa

Applications for Glycogen Synthase [p Ser641] Antibody (SR46-06)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000
Application Notes
IP: Use at an assay dependent concentration.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glycogen Synthase

Glycogen synthase is an enzyme of the transferase class that catalyses the reaction of UDP-glucose and (1,4-

Alternate Names

EC 2.4.1.11, glycogen [starch] synthase, muscle, glycogen synthase 1 (muscle), GYSGSY

Gene Symbol

GYS1

Additional Glycogen Synthase Products

Product Documents for Glycogen Synthase [p Ser641] Antibody (SR46-06)

Certificate of Analysis

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Product Specific Notices for Glycogen Synthase [p Ser641] Antibody (SR46-06)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Glycogen Synthase [p Ser641] Antibody (SR46-06)

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Protocols

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