GRK2 Antibody (23GB1515)

Novus Biologicals | Catalog # NBP3-51786

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB1515
View all GRK2 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human GRK2 (Uniprot: P25098)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRK2 Antibody (23GB1515)

GRK2 Antibody (23GB1515)

Immunocytochemistry/ Immunofluorescence: GRK2 Antibody (23GB1515)[NBP3-51786] -

GRK2 antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: GRK2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; GRK2 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um. Permeabilization: Triton.
GRK2 Antibody (23GB1515)

Immunocytochemistry/ Immunofluorescence: GRK2 Antibody (23GB1515)[NBP3-51786] -

Immunocytochemical staining of HepG2 cells with GRK2 antibody (1:1,000). Nuclei were stained blue with DAPI; GRK2 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
GRK2 Antibody (23GB1515)

Flow Cytometry: GRK2 Antibody (23GB1515)[NBP3-51786] -

Validation of GRK2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with GRK2 antibody (1:2,000) and analyzed using BD flow cytometer.
GRK2 Antibody (23GB1515)

Flow Cytometry: GRK2 Antibody (23GB1515)[NBP3-51786] -

Flow cytometric analysis of GRK2expression in HepG2 cells using GRK2 antibody (1:2,000). Green, isotype control; red, GRK2.
GRK2 Antibody (23GB1515)

Western Blot: GRK2 Antibody (23GB1515)[NBP3-51786] -

Western blotting analysis using GRK2 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.
GRK2 Antibody (23GB1515)

Western Blot: GRK2 Antibody (23GB1515)[NBP3-51786] -

Western blotting analysis using GRK2 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for GRK2 Antibody (23GB1515)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GRK2

Adrenergic Receptor beta Kinase 1 (ADRBK1), also known as GRK2, is a member of the G-protein receptor kinase family. A ubiquitous cytosolic enzyme, GRK-2 specifically phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed under high agonist concentrations. Defects in GRK2 coupling with G-protein receptors may lead to heart failure.

Long Name

G protein-coupled Receptor Kinase 2

Alternate Names

ADRBK1, BARK1, Beta-ARK1

Gene Symbol

GRK2

Additional GRK2 Products

Product Documents for GRK2 Antibody (23GB1515)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GRK2 Antibody (23GB1515)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases

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Protocols

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FAQs

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