HARS Antibody (24GB6755)

Novus Biologicals | Catalog # NBP3-51791

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 24GB6755
View all HARS Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein of human HARS (Uniprot: P12081)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HARS Antibody (24GB6755)

HARS Antibody (24GB6755)

Immunocytochemistry/ Immunofluorescence: HARS Antibody (24GB6755)[NBP3-51791] -

HARS antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: HARS1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; HARS1 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um. Permeabilization: Triton.
HARS Antibody (24GB6755)

Immunocytochemistry/ Immunofluorescence: HARS Antibody (24GB6755)[NBP3-51791] -

Immunocytochemical staining of HepG2 cells with HARS antibody (1:1,000). Nuclei were stained blue with DAPI; HARS was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
HARS Antibody (24GB6755)

Flow Cytometry: HARS Antibody (24GB6755)[NBP3-51791] -

Flow cytometric analysis of HARSexpression in HepG2 cells using HARS antibody (1:2,000). Green, isotype control; red, HARS1.
HARS Antibody (24GB6755)

Flow Cytometry: HARS Antibody (24GB6755)[NBP3-51791] -

Validation of HARS knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with HARS antibody (1:2,000) and analyzed using BD flow cytometer.
HARS Antibody (24GB6755)

Western Blot: HARS Antibody (24GB6755)[NBP3-51791] -

Western blotting analysis using HARS antibody (1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.

Applications for HARS Antibody (24GB6755)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HARS

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.

Alternate Names

cytoplasmic, EC 6.1.1.21, FLJ20491, histidine-tRNA ligase, histidyl-tRNA synthetase, histidyl-tRNA synthetase, cytoplasmic

Gene Symbol

HARS1

Additional HARS Products

Product Documents for HARS Antibody (24GB6755)

Certificate of Analysis

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Product Specific Notices for HARS Antibody (24GB6755)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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