HDAC6 Antibody (236) - BSA Free

Novus Biologicals | Catalog # NBP3-05295

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse (Negative), Zebrafish (Negative)

Applications

Western Blot, ELISA, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse Clone # 236

Format

BSA Free
View all Histone Deacetylase 6/HDAC6 Primary Antibodies »

Product Specifications

Immunogen

Human HDAC6

Specificity

The mouse monoclonal antibody 236 recognizes D1 domain of human histone deacetylase 6 (HDAC6; intracellular antigen), amino acids 71-91. Crossreactivity with other species was not tested.

Clonality

Monoclonal

Host

Mouse

Scientific Data Images for HDAC6 Antibody (236) - BSA Free

Western Blot: HDAC6 Antibody (236) [NBP3-05295]

Western Blot: HDAC6 Antibody (236) [NBP3-05295]

Western Blot: HDAC6 Antibody (236) [NBP3-05295] - Western blotting analysis of human HDAC6 by mouse monoclonal antibody NBP3-05295 in HEK-293T cell line under reducing conditions. PVDF membrane was blocked in 5% milk, and incubated with primary antibody (1 ug/ml), followed by HRP-conjugated anti-mouse secondary antibody.
Immunocytochemistry/ Immunofluorescence: HDAC6 Antibody (236) [NBP3-05295]

Immunocytochemistry/ Immunofluorescence: HDAC6 Antibody (236) [NBP3-05295]

Immunocytochemistry/Immunofluorescence: HDAC6 Antibody (236) [NBP3-05295] - Immunocytochemistry staining of HDAC6 in formaldehyde-fixed and Triton-permeabilized HEK-293T cells (A) and SH-SY5Y cells (B) by mouse monoclonal antibody NBP3-05295, followed by anti-mouse Alexa Fluor 488 (green), DNA indicated by DAPI (blue). In SH-SY5Y cells tubulin was stained by a rabbit polyclonal antibody, followed by anti-rabbit Alexa Fluor 647 (red, colocalization yellow).
ELISA: HDAC6 Antibody (236) [NBP3-05295]

ELISA: HDAC6 Antibody (236) [NBP3-05295]

ELISA: HDAC6 Antibody (236) [NBP3-05295] - ELISA analysis of human HDAC6 by mouse monoclonal antibody NBP3-05295. Plate was coated with recombinant human HDAC6, then blocked with BSA, and exposed to dilution series of anti-HDAC6 primary antibody, followed by HRP-conjugated anti-mouse secondary antibody, and colorimetric signal of processed OPD substrate was measured at 492 nm.
Immunoprecipitation: HDAC6 Antibody (236) [NBP3-05295]

Immunoprecipitation: HDAC6 Antibody (236) [NBP3-05295]

Immunoprecipitation: HDAC6 Antibody (236) [NBP3-05295] - Immunoprecipitation of recombinant human HDAC6 by mouse monoclonal antibodies 178 and NBP3-05295 using protein G-coated Dynabeads. Eluted proteins were processed by SDS PAAGE under reducing conditions and visualized by Coomassie Brilliant Blue.
HDAC6 Antibody (236)

Flow Cytometry: Mouse Monoclonal HDAC6 Antibody (236) [NBP3-05295] -

Flow Cytometry: Mouse Monoclonal HDAC6 Antibody (236) [NBP3-05295] - Separation of K562 cells stained using anti-human HDAC6 (236) purified antibody (concentration in sample 5.33 ug/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

Applications for HDAC6 Antibody (236) - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Histone Deacetylase 6/HDAC6

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq]

Long Name

Histone Deacetylase 6

Alternate Names

CPBHM, FLJ16239, HD6, HDAC6, JM21, PPP1R90

Gene Symbol

HDAC6

Additional Histone Deacetylase 6/HDAC6 Products

Product Documents for HDAC6 Antibody (236) - BSA Free

Certificate of Analysis

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Product Specific Notices for HDAC6 Antibody (236) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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