HES-1 Antibody (9I2E8)
Novus Biologicals | Catalog # NBP3-15320
Recombinant Monoclonal Antibody
![Western Blot: HES-1 Antibody (9I2E8) [NBP3-15320]](https://resources.rndsystems.com/images/products/HES-1-Antibody-9I2E8-Western-Blot-NBP3-15320-img0003.jpg "Western Blot: HES-1 Antibody (9I2E8) [NBP3-15320]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Species Reactivity
Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 9I2E8 expressed in HEK293
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Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 181-280 of human HES-1 (Q14469). FAPPPPLVPIPGGAAPPPGGAPCKLGSQAGEAAKVFGGFQVVPAPDGQFAFLIPNGAFAHSGPVIPVYTSNSGTSVGPNAVSPSSGPSLTADSMWRPWRN
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HES-1 Antibody (9I2E8)
Western Blot: HES-1 Antibody (9I2E8) [NBP3-15320]
Western Blot: HES-1 Antibody (9I2E8) [NBP3-15320] - Western blot analysis of extracts of various cell lines, using HES-1 Rabbit mAb (NBP3-15320) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.![Immunohistochemistry-Paraffin: HES-1 Antibody (9I2E8) [NBP3-15320]](https://resources.rndsystems.com/images/products/HES-1-Antibody-9I2E8-Immunohistochemistry-Paraffin-NBP3-15320-img0002.jpg "Immunohistochemistry-Paraffin: HES-1 Antibody (9I2E8) [NBP3-15320]")
Immunohistochemistry-Paraffin: HES-1 Antibody (9I2E8) [NBP3-15320]
Immunohistochemistry-Paraffin: HES-1 Antibody (9I2E8) [NBP3-15320] - Immunohistochemistry of paraffin-embedded mouse spinal cord using HES-1 Rabbit mAb (NBP3-15320) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.![Immunohistochemistry-Paraffin: HES-1 Antibody (9I2E8) [NBP3-15320]](https://resources.rndsystems.com/images/products/HES-1-Antibody-9I2E8-Immunohistochemistry-Paraffin-NBP3-15320-img0001.jpg "Immunohistochemistry-Paraffin: HES-1 Antibody (9I2E8) [NBP3-15320]")
Immunohistochemistry-Paraffin: HES-1 Antibody (9I2E8) [NBP3-15320]
Immunohistochemistry-Paraffin: HES-1 Antibody (9I2E8) [NBP3-15320] - Immunohistochemistry of paraffin-embedded rat brain using HES-1 Rabbit mAb (NBP3-15320) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.![Immunoprecipitation: HES-1 Antibody (9I2E8) [NBP3-15320]](https://resources.rndsystems.com/images/products/HES-1-Antibody-9I2E8-Immunoprecipitation-NBP3-15320-img0004.jpg "Immunoprecipitation: HES-1 Antibody (9I2E8) [NBP3-15320]")
Immunoprecipitation: HES-1 Antibody (9I2E8) [NBP3-15320]
Immunoprecipitation: HES-1 Antibody (9I2E8) [NBP3-15320] - Immunoprecipitation analysis of 600ug extracts of Rat testis cells using 3ug HES-1 antibody (NBP3-15320). Western blot was performed from the immunoprecipitate using HES-1 antibody (NBP3-15320) at a dilition of 1:500.
Immunohistochemistry: HES-1 Antibody (9I2E8) [HES-1] -
Immunohistochemistry: HES-1 Antibody (9I2E8) [HES-1] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using HES-1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry: HES-1 Antibody (9I2E8) [HES-1] -
Immunohistochemistry: HES-1 Antibody (9I2E8) [HES-1] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using HES-1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry: HES-1 Antibody (9I2E8) [HES-1] -
Immunohistochemistry: HES-1 Antibody (9I2E8) [HES-1] - Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using HES-1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry: HES-1 Antibody (9I2E8) [HES-1] -
Immunohistochemistry: HES-1 Antibody (9I2E8) [HES-1] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using HES-1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry: HES-1 Antibody (9I2E8) [HES-1] -
Immunohistochemistry: HES-1 Antibody (9I2E8) [HES-1] - Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using HES-1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.Applications for HES-1 Antibody (9I2E8)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunoprecipitation
1:100 - 1:500
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HES-1
Long Name
Hairy And Enhancer of Split 1
Alternate Names
HES1, HL, HRY
Gene Symbol
HES1
Additional HES-1 Products
Product Documents for HES-1 Antibody (9I2E8)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HES-1 Antibody (9I2E8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
