HIP1 Antibody (1B11) - BSA Free

Novus Biologicals | Catalog # NB300-204

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bacteria, Rat (Negative)

Cited:

Human, Mouse, Bacteria

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1B11

Format

BSA Free
View all HIP1 Primary Antibodies »

Product Specifications

Immunogen

Human 3'-HIP1 (~65 kDa)

Reactivity Notes

Does not appear to work in rat.

Localization

Localization: Cytoplasmic

Specificity

NB300-204 is specific for human HIP1. Does not cross-react with HIP1r.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

115 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HIP1 Antibody (1B11) - BSA Free

Western Blot: HIP1 Antibody (1B11) [NB300-204]

Western Blot: HIP1 Antibody (1B11) [NB300-204]

Western Blot: HIP1 Antibody (1B11) [NB300-204] - HeLa whole cell extracts.
Immunocytochemistry/ Immunofluorescence: HIP1 Antibody (1B11) [NB300-204]

Immunocytochemistry/ Immunofluorescence: HIP1 Antibody (1B11) [NB300-204]

Immunocytochemistry/Immunofluorescence: HIP1 Antibody (1B11) [NB300-204] - Human pluripotent embryonal carcinoma cells stained with HIP1 antibody. ICC/IF image submitted by a verified customer review.

Applications for HIP1 Antibody (1B11) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunoprecipitation

reported in scientific literature (PMID 23272104)

Western Blot

1:1000
Application Notes
In Western blot, a band is observed at ~115 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 5 using NB300-204 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HIP1

One of the four subunits of the histone regulatory complex A, HIP1 is an anti-apoptosis protein in the brain. HIP1 (Huntingtin-interacting protein 1) protects normal neurological tissue from erroneous inflammatory and apoptotic events by linking the actin cytoskeleton and clathrin-mediated endocytosis machinery. It is important in the generation of clathrin coated vesicles in the neuron. However, if deletion of HIP1 occurs, neurological disease is initiated and is correlated to epilepsy, learning disabilities, spinal and testicular defects, hematopoietic issues, and other neurobehavioral abnormalities such as Huntington's disease. HIP1 also interacts with the Hedgehog signaling pathway, and is important in regulating the abundance and scope of Hedgehog signaling.

Alternate Names

HIP-1, HIP-I, huntingtin interacting protein 1, huntingtin-interacting protein 1, Huntingtin-interacting protein I, ILWEQ, MGC126506

Entrez Gene IDs

3092 (Human); 192154 (Rat)

Gene Symbol

HIP1

UniProt

O00291 (Human)

Additional HIP1 Products

Product Documents for HIP1 Antibody (1B11) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for HIP1 Antibody (1B11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HIP1 Antibody (1B11) - BSA Free

Customer Reviews for HIP1 Antibody (1B11) - BSA Free (1)

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  • HIP1 Antibody (1B11)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Human pluripotent embryonal carcinoma cells
    Species: Human
    Verified Customer | Posted 11/11/2021
    HIP1 Antibody. Human pluripotent embryonal carcinoma cells.
    HIP1 Antibody (1B11) - BSA Free NB300-204

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Protocols

View specific protocols for HIP1 Antibody (1B11) - BSA Free (NB300-204):

HIP1 Antibody (1B11):
Western Blot Procedure

1. Run 25 ug/lane of protein (ie: HeLa whole cell extract) on a 10% SDS-PAGE gel.
2. Transfer the proteins to nitrocellulose.
3. Block the membrane with a standard blocking buffer (5% NFDM in TBST), overnight at 4 deg C.
4. Dilute the anti-HIP1 in 1% NFDM in TBST and incubate for 1hour at RT.
5. Wash the membrane, 3x 5-10 minutes.
6. Dilute the secondary antibody [anti-mouse IgG conjugated to HRP] in 1% NFDM in TBST and incubate for 1hour at RT.
7. Wash the membrane, 3x 5-10 minutes.
8. Detect the protein-antibody complex via ECL.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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