His Tag Antibody (3D5) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-52637

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Epitope Tag

Applications

Western Blot, ELISA, Simple Western

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 3D5

Format

Azide and BSA Free
View all His Tag Primary Antibodies »

Product Specifications

Immunogen

This His Tag Antibody (3D5) - Chimeric was developed against purified his tagged Fab fragment.

Specificity

This antibody binds to carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins. It also has affinity for internal His-tags but no affinity for N-terminal His-tags.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for His Tag Antibody (3D5) - Chimeric - Azide and BSA Free

Simple Western: His Tag Antibody (3D5)ChimericAzide and BSA Free [NBP2-52637]

Simple Western: His Tag Antibody (3D5)ChimericAzide and BSA Free [NBP2-52637]

Simple Western: His Tag Antibody (3D5) - Chimeric [NBP2-52637] - His Tag Antibody (3D5) [NBP2-52637] - Human purified protein. Simple Western image submitted by a verified customer review.
His Tag Antibody (3D5) - Chimeric - Azide and BSA Free

Western Blot: His Tag Antibody (3D5) - Chimeric - Azide and BSA Free [NBP2-52637] -

Western Blot: His Tag Antibody (3D5) - Chimeric - Azide and BSA Free [NBP2-52637] - His tag expressing plasmid transfected (A) and non-transfected (B) HEK293 cells lysate (3g protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with NBP2-52637 at 0.01ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
His Tag Antibody (3D5) - Chimeric - Azide and BSA Free

Immunocytochemistry /Immunofluorescence: His Tag Antibody (3D5) - Chimeric - Azide and BSA Free [NBP2-52637] -

Immunocytochemistry /Immunofluorescence: His Tag Antibody (3D5) - Chimeric - Azide and BSA Free [NBP2-52637] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells transfected with His tag expressing plasmid (A) and non-transfected HEK293 cells (B), permeabilized with 0.15% Triton stained with NBP2-52637 (1:200 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue).

Applications for His Tag Antibody (3D5) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Western Blot

1:100 - 1:2000
Application Notes
This is a chimeric rabbit antibody, based on the original mouse IgG2b format, created for improved compatibility with existing reagents, assays and techniques.
See Simple Western Antibody Database for Simple Western validation: Tested in Human purified protein, separated by Size

Reviewed Applications

Read 1 review rated 5 using NBP2-52637 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: His Tag

A his-tag (also known as histidine tag or polyhistidine tag) is a common epitope tag that typically consists of at least 6 histidine residues fused to either the carboxyl (C-) or amino (N-) terminus of a targeted recombinant protein to facilitate its purification and detection (1). The most common his-tag is the hexahistidine (His6/6-His) tag which has a theoretical molecular weight of 0.8kda (1). The histidine residues readily interact with transition metal ions such as Co2+, Ni2+, Cu2+, and Zn2+, making immobilized metal-affinity chromatography (IMAC) the preferred technique for his-tag purification (1, 2). Metal ions are immobilized and bound to by His-tags in the IMAC column via the histidine imidazole ring. The tagged protein can be eluted off the column by washing with buffers containing a low concentration of imidazole (1, 2). Due to its relatively small size, low immunogenicity, versatility under denaturing conditions, and minimal interference with the structure and function of proteins, the his-tag is one of the most widely used tags for protein purification (1-3).

References

1. Malhotra, A. (2009). Tagging for protein expression. Methods in Enzymology, Guide to Protein Purification, 2nd Edition, 463, 239-258. https://doi.org/10.1016/s0076-6879(09)63016-0

2. Terpe, K. (2003). Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology, 60(5), 523-533. https://doi.org/10.1007/s00253-002-1158-6

3. Booth, W. T., Schlachter, C. R., Pote, S., Ussin, N., Mank, N. J., Klapper, V.,... Chruszcz, M. (2018). Impact of an N-terminal polyhistidine tag on protein thermal stability. ACS Omega, 3(1), 760-768. https://doi.org/10.1021/acsomega.7b01598

Long Name

Histidine Tag

Alternate Names

polyHistidine

Additional His Tag Products

Product Documents for His Tag Antibody (3D5) - Chimeric - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for His Tag Antibody (3D5) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for His Tag Antibody (3D5) - Chimeric - Azide and BSA Free (1)

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  • His Tag Antibody (3D5)
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Purified protein
    Species: Human
    Verified Customer | Posted 12/04/2018
    His Tag Antibody (3D5) - Chimeric - Azide and BSA Free NBP2-52637

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