Histone H1.2 Antibody (JU43-48)

Novus Biologicals | Catalog # NBP2-75932

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JU43-48 expressed in HEK293
View all Histone H1.2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Histone H1.2 aa 1-50 / 213. (SwissProt: P16403 Human; SwissProt: P15864 Mouse; SwissProt: P15865 Rat)

Localization

Nucleus. Chromosome.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Histone H1.2 Antibody (JU43-48)

Western Blot: Histone H1.2 Antibody (JU43-48) [NBP2-75932]

Western Blot: Histone H1.2 Antibody (JU43-48) [NBP2-75932]

Western Blot: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - analysis of Histone H1.2 on Rat liver and Mouse lung tissue lysates using anti-Histone H1.2 antibody at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence: Histone H1.2 Antibody (JU43-48) [NBP2-75932]

Immunocytochemistry/ Immunofluorescence: Histone H1.2 Antibody (JU43-48) [NBP2-75932]

Immunocytochemistry/Immunofluorescence: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - staining Histone H1.2 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Histone H1.2 Antibody (JU43-48) [NBP2-75932]

Immunohistochemistry-Paraffin: Histone H1.2 Antibody (JU43-48) [NBP2-75932]

Immunohistochemistry-Paraffin: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Histone H1.2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western Blot: Histone H1.2 Antibody (JU43-48) [NBP2-75932]

Western Blot: Histone H1.2 Antibody (JU43-48) [NBP2-75932]

Western Blot: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - analysis of Histone H1.2 on different cell lysates using anti-Histone H1.2 antibody at 1/500 dilution.Positive control:Lane 1: Hela Lane 2: 293 Lane 3: MCF-7
Immunocytochemistry/ Immunofluorescence: Histone H1.2 Antibody (JU43-48) [NBP2-75932]

Immunocytochemistry/ Immunofluorescence: Histone H1.2 Antibody (JU43-48) [NBP2-75932]

Immunocytochemistry/Immunofluorescence: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - staining Histone H1.2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: Histone H1.2 Antibody (JU43-48) [NBP2-75932]

Immunocytochemistry/ Immunofluorescence: Histone H1.2 Antibody (JU43-48) [NBP2-75932]

Immunocytochemistry/Immunofluorescence: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - staining Histone H1.2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Histone H1.2 Antibody (JU43-48) [NBP2-75932]

Immunohistochemistry-Paraffin: Histone H1.2 Antibody (JU43-48) [NBP2-75932]

Immunohistochemistry-Paraffin: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - analysis of paraffin-embedded mouse colon tissue using anti-Histone H1.2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Histone H1.2 Antibody (JU43-48) [NBP2-75932]

Immunohistochemistry-Paraffin: Histone H1.2 Antibody (JU43-48) [NBP2-75932]

Immunohistochemistry-Paraffin: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - analysis of paraffin-embedded human kidney tissue using anti-Histone H1.2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Histone H1.2 Antibody (JU43-48) [NBP2-75932]

Immunohistochemistry-Paraffin: Histone H1.2 Antibody (JU43-48) [NBP2-75932]

Immunohistochemistry-Paraffin: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - analysis of paraffin-embedded mouse colon tissue using anti-Histone H1.2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. Counter stained with hematoxylin.

Applications for Histone H1.2 Antibody (JU43-48)

Application
Recommended Usage

Chromatin Immunoprecipitation

1:10-1:500

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H1.2

Histones are nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber as they play a critical role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. The Histone H1.2 protein is 213 amino acids long, 21 kDA and is encoded by the HIST1H1C gene. H1 proteins are essential in forming chromatin fiber and for the condensation of nucleosome chains into higher-order structured fibers. The HIST1H1C gene has been investigated in leukemia and parkinson's disease. It plays a role in apoptosis, PKA signaling, cell cycle chromosome condensation in prometaphase, and histone modification through interactions with various genes such as TONSL, NPM1, YWHAQ, EED, and ESR2.

Alternate Names

H1 histone family, member 2, H1.2, H1F2H1C, histone 1, H1c, histone cluster 1, H1c, histone H1.2, Histone H1d, MGC3992

Gene Symbol

H1-2

Additional Histone H1.2 Products

Product Documents for Histone H1.2 Antibody (JU43-48)

Certificate of Analysis

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Product Specific Notices for Histone H1.2 Antibody (JU43-48)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Histone H1.2 Antibody (JU43-48)

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