Histone H2AX Antibody - BSA Free
Novus Biologicals | Catalog # NB100-383
![Western Blot: Histone H2AX Antibody [NB100-383]](https://resources.rndsystems.com/images/products/Histone-H2AX-Antibody-Western-Blot-NB100-383-img0043.jpg "Western Blot: Histone H2AX Antibody [NB100-383]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to the C-terminus of human histone H2AX using the numbering given for Swiss-Prot entry P16104 (GeneID 3014).
Specificity
This is specific for H2AX. It is not known if this also recognizes the phosphorylated form of H2AX.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H2AX Antibody - BSA Free
Western Blot: Histone H2AX Antibody [NB100-383]
Western Blot: Histone H2AX Antibody [NB100-383] - Whole cell lysate (50 ug) from HEK293T cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-H2AX antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.![Immunohistochemistry-Paraffin: Histone H2AX Antibody [NB100-383]](https://resources.rndsystems.com/images/products/Histone-H2AX-Antibody-Immunohistochemistry-NB100-383-img0040.jpg "Immunohistochemistry-Paraffin: Histone H2AX Antibody [NB100-383]")
Immunohistochemistry-Paraffin: Histone H2AX Antibody [NB100-383]
Immunohistochemistry-Paraffin: Histone H2AX Antibody [NB100-383] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-H2AX (NB100-383). Detection: DAB![Western Blot: Histone H2AX Antibody [NB100-383]](https://resources.rndsystems.com/images/products/Histone-H2AX-Antibody-Simple-Western-NB100-383-img0039.jpg "Western Blot: Histone H2AX Antibody [NB100-383]")
Western Blot: Histone H2AX Antibody [NB100-383]
Western Blot: Histone H2AX Antibody [NB100-383] - Detection of mouse H2AX by western blot. Samples: Whole cell lysate (50 ug) from F9 and L1210 cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-H2AX antibody A300-083A (lot A300-083A-5) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Simple Western: Histone H2AX Antibody [NB100-383]](https://resources.rndsystems.com/images/products/Histone-H2AX-Antibody-Simple-Western-NB100-383-img0031.jpg "Simple Western: Histone H2AX Antibody [NB100-383]")
Simple Western: Histone H2AX Antibody [NB100-383]
Simple Western: Histone H2AX Antibody [NB100-383] - Simple Western lane view shows a specific band for H2AX in 0.2 mg/ml of HEK lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.![Simple Western: Histone H2AX Antibody [NB100-383]](https://resources.rndsystems.com/images/products/Histone-H2AX-Antibody-Simple-Western-NB100-383-img0032.jpg "Simple Western: Histone H2AX Antibody [NB100-383]")
Simple Western: Histone H2AX Antibody [NB100-383]
Simple Western: Histone H2AX Antibody [NB100-383] - Electropherogram image(s) of corresponding Simple Western lane view. H2AX antibody was used at 10 ug/ml dilution on HEK lysate(s).Applications for Histone H2AX Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:250-1:1000
Immunohistochemistry-Paraffin
1:500-1:2000
Simple Western
10 ug/ml
Western Blot
1:2000-1:10000
Application Notes
By Western blot, this antibody recognizes a band at ~25 kDa, representing monoubiquinated H2AX protein and ~14 kDa, representing H2AX. IP, IHC reported in (PMID: 22426396). ICC/IF reported in (PMID: 20203688). br/>In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HEK lysate, separated by Size, antibody dilution of 10 ug/mL, apparent MW was 28 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
See Simple Western Antibody Database for Simple Western validation: Tested in HEK lysate, separated by Size, antibody dilution of 10 ug/mL, apparent MW was 28 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Histone H2AX
Additional Histone H2AX Products
Product Documents for Histone H2AX Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Histone H2AX Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Histone H2AX Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Histone H2AX Antibody - BSA Free
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Q: Could you provide the detailed immunogen peptide sequence?
A: The epitope which is recognized by NB100-383 maps to the C-terminus of human histone H2AX (Swiss-Prot entry P16104). Unfortunately we are not able to provide any further information than this as the exact immunogen information is considered to be proprietary.
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