Histone H2B type 2E [Crotonyl Lys12] Antibody (23GB6335)

Name: Histone H2B type 2E [Crotonyl Lys12] Antibody (23GB6335)
Brand: Novus Biologicals
SKU: NBP3-50477
Price: 379 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-50477

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB6335

View all Histone H2B type 2E Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Histone H2B type 2E (crotonyl K12) (Uniprot: Q16778)

Modification

Crotonyl Lys12

Specificity

This antibody detects endogenous levels of H2BC21 only when Crotonylated at K12

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H2B type 2E [Crotonyl Lys12] Antibody (23GB6335)

Immunocytochemistry/ Immunofluorescence: Histone H2B type 2E [Crotonyl Lys12] Antibody (23GB6335)[NBP3-50477] -

Immunocytochemical staining of HepG2 cells with Histone H2B type 2E antibody (1:1,000). Nuclei were stained blue with DAPI; Histone H2B type 2E was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.

Western Blot: Histone H2B type 2E [Crotonyl Lys12] Antibody (23GB6335)[NBP3-50477] -

Western blotting analysis using Histone H2B type 2E (crotonyl K12) antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for Histone H2B type 2E [Crotonyl Lys12] Antibody (23GB6335)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone H2B type 2E

Alternate Names

GL105, H2B, H2B Histone Family, Member Q, H2B.1, Histone 2, H2be, Histone Cluster 2 H2B Family Member E, Histone Cluster 2, H2be, Histone H2B Type 2-E, Histone H2B.Q, Histone H2B-GL105

Gene Symbol

H2BC21

Additional Histone H2B type 2E Products

Product Documents for Histone H2B type 2E [Crotonyl Lys12] Antibody (23GB6335)

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Certificate of Analysis

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Product Specific Notices for Histone H2B type 2E [Crotonyl Lys12] Antibody (23GB6335)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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