Viral HIV-1 Gag p24 Antibody (749140)

R&D Systems | Catalog # MAB7360

R&D Systems

Key Product Details

Species Reactivity

Validated:

Viral

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 749140
View all HIV-1 Gag p24 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant viral HIV-1 Gag p24
Pro149-Gly354
Accession # NP_057850

Specificity

Detects viral HIV-1 Gag p24 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Viral HIV-1 Gag p24 Antibody (749140)

Detection of HIV-1 Gag p24 antibody by Western Blot.

Detection of HIV-1 Gag p24 by Western Blot.

Western blot shows recombinant HIV Gag p24 (5 ng/lane). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Viral HIV-1 Gag p24 Monoclonal Antibody (Catalog # MAB7360) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HIV-1 Gag p24 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of HIV-1 Gag p24 by Western Blot

Detection of HIV-1 Gag p24 by Western Blot

Effect of ZCCHC3 on viral production(A) Effect of ZCCHC3 on HIV-1 viral production. A plasmid encoding HA-tagged ZCCHC3 or empty HA-vector was introduced into Lenti-X 293T cells together with an HIV-1-encoding plasmid. Virions released into the culture medium were quantified by p24 ELISA. The absolute value (top) and the value relative to that without ZCCHC3 (bottom) are shown (n = 3).(B) ZCCHC3 affects the Gag processing. Lenti-X 293T cells were co-transfected with pTF2625 plasmids in the presence or absence of HA-ZCCHC3. Equal volumes of the pelleted virions and cell lysates were analyzed via western blotting using anti-p24 and anti-HA antibodies. The total protein in the cell lysate was also analyzed via CBB staining.(C) Effect of ZCCHC3 on infectivity of ZCCHC3-loaded virions. TZM-bl cells were infected with the same amount (p24-normalized) of HIV-1NL4-3 or lentivirus with or without ZCCHC3 in the virion. Infectivity was analyzed by luciferase assay and is presented relative to that without ZCCHC3 as the mean and standard deviation (n = 5).(D) Effect of ZCCHC3 co-expression on lentivirus production. HEK293T cells were transfected with lentiviral plasmids (pLV-EGFP, psPAX2, and pMD2.G) with or without an HA-ZCCHC3 expression plasmid. Total RNA was purified from lentiviruses harvested from the culture medium and analyzed by RT-qPCR. The mean and standard deviation values are shown (n = 3). In (A), (C), and (D), differences were examined by a two-tailed, unpaired Student’s t test; ∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, ∗∗p < 0.001, ∗p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://linkinghub.elsevier.com/retrieve/pii/S2589004224003286), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Viral HIV-1 Gag p24 Antibody (749140)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Recombinant HIV Gag p24

Reviewed Applications

Read 2 reviews rated 4.5 using MAB7360 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HIV-1 Gag p24

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.

Long Name

Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa

Alternate Names

CA, Capsid Protein, HIV1 Gag p24, Pr55(Gag)

Entrez Gene IDs

155030 (Viral)

Gene Symbol

GAG

UniProt

NP_057850

Additional HIV-1 Gag p24 Products

Product Documents for Viral HIV-1 Gag p24 Antibody (749140)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Viral HIV-1 Gag p24 Antibody (749140)

For research use only

Related Research Areas

Infectious Diseases

Citations for Viral HIV-1 Gag p24 Antibody (749140)

Customer Reviews for Viral HIV-1 Gag p24 Antibody (749140) (2)

4.5 out of 5
2 Customer Ratings
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  • HIV-1 Gag p24 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: HIV
    Verified Customer | Posted 10/09/2018
    gag protein w/o HIV protease, expressed in HEK293 cells, 1/500 dilution
    Viral HIV-1 Gag p24 Antibody (749140) MAB7360
  • HIV-1 Gag p24 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Cell Lysates
    Species: Viral
    Verified Customer | Posted 02/24/2016

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