HR6B/UBE2B Antibody

Novus Biologicals | Catalog # NBP1-31293

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Porcine (100%), Rabbit (100%), Rat (100%), Rhesus Macaque (100%), Xenopus (98%), Zebrafish (95%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all HR6B/UBE2B Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human HR6B/UBE2B. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (98%).

Localization

Cell membrane, Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HR6B/UBE2B Antibody

Western Blot: HR6B/UBE2B Antibody [NBP1-31293]

Western Blot: HR6B/UBE2B Antibody [NBP1-31293]

Western Blot: HR6B/UBE2B Antibody [NBP1-31293] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2B antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: HR6B/UBE2B Antibody [NBP1-31293]

Immunocytochemistry/ Immunofluorescence: HR6B/UBE2B Antibody [NBP1-31293]

Immunocytochemistry/Immunofluorescence: HR6B/UBE2B Antibody [NBP1-31293] - Paraformaldehyde-fixed A431, using antibody at 1:500 dilution.
Western Blot: HR6B/UBE2B Antibody [NBP1-31293]

Western Blot: HR6B/UBE2B Antibody [NBP1-31293]

Western Blot: HR6B/UBE2B Antibody [NBP1-31293] - A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of 3xFlag-human UBE2B-transfected 293T cells. Used at a dilution of 1:5000.

Applications for HR6B/UBE2B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HR6B/UBE2B

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.

Long Name

Ubiquitin-conjugating Enzyme E2B

Alternate Names

E2-17k, UBE2B

Gene Symbol

UBE2B

Additional HR6B/UBE2B Products

Product Documents for HR6B/UBE2B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HR6B/UBE2B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Epigenetics
p53 Pathway

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Ubiquitination Cascade Pathway
Ubiquitination Cascade Pathway Thumbnail