Human ADAMTS13 Antibody [Biotin]

R&D Systems | Catalog # BAF4245

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all ADAMTS13 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS13
Gln34-Trp688
Accession # Q76LX8

Specificity

Detects human ADAMTS13 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) ADAMTS1, rhADAMTSL-1.2, rhADAMTS4, and rhADAMTS5 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human ADAMTS13 Antibody [Biotin]

Human ADAMTS13 ELISA Standard Curve

Human ADAMTS13 ELISA Standard Curve

Recombinant Human ADAMTS13 Full Length (Catalog # 6156-AD) was serially diluted and captured by Mouse Anti-Human ADAMTS13 Monoclonal Antibody (Catalog # MAB42451) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human ADAMTS13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4245) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human ADAMTS13 Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human ADAMTS13 (Catalog # 4245-AD)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ADAMTS13

ADAMTS13 (a disintegrin and metalloproteinase with thrombospondin motifs 13) is a secreted multi-domain zinc protease primarily produced by the liver. In the circulation, it is responsible for cleaving von Willebrand factor (vWF) between Tyr842 and Met843. Defects in ADAMTS13 activity result in the generation of ultra large vWF, leading to thrombotic thrombocytopenic purpura (TTP). The amino acid sequence of human ADAMTS13 in the region of aa 34 to 688 is 76% identical to that of mouse/rat.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 13

Alternate Names

TTP, vWF-CP

Entrez Gene IDs

11093 (Human); 279028 (Mouse); 362091 (Rat)

Gene Symbol

ADAMTS13

UniProt

Q76LX8

Additional ADAMTS13 Products

Product Documents for Human ADAMTS13 Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ADAMTS13 Antibody [Biotin]

For research use only

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