Human Adiponectin/Acrp30 Antibody (553517)

R&D Systems | Catalog # MAB10652

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 553517
View all Adiponectin/Acrp30 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Adiponectin/Acrp30
Glu19-Asn244
Accession # Q15848

Specificity

Detects human Adiponectin/Acrp30 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Acrp30 (aa 101-244), recombinant mouse (rm) Acrp30 (aa 111-247), rmAcrp30 (HWM), recombinant rat Acrp30 (aa 1-244), rhC1qTNF1, rmCORS26/C1qTNF3, rhC1qTNF4, rhC1qTNF5, rhC1qTNF9, or rhC1qL2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Adiponectin/Acrp30 Antibody (553517)

Detection of Human Adiponectin/Acrp30 antibody by Western Blot.

Detection of Human Adiponectin/Acrp30 by Western Blot.

Western blot shows lysates of human adipose tissue. PVDF Membrane was probed with 2 µg/mL of Human Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB10652) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Adiponectin/Acrp30 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Adiponectin/Acrp30 Antibody (553517)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human adipose tissue

Reviewed Applications

Read 1 review rated 5 using MAB10652 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Adiponectin/Acrp30

Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It is induced during adipocyte differentiation, and its secretion is stimulated by insulin. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, Adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in non-metabolic disorders such as rheumatoid arthritis and inflammatory bowel disease (1‑3). Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin 13/T-Cadherin, as well as with several growth factors (4‑7). Mature human Adiponectin consists of a 60 amino acid (aa) N-terminal collagenous region and a 137 aa C-terminal C1q-like globular domain which can be cleaved by a leukocyte-derived elastase (8, 9). Mature human Adiponectin shares 83% and 85% amino acid (aa) sequence identity with mouse and rat Adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into > 300 kDa high molecular weight (HMW) oligomers (10‑12). The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes (13). The various multimeric forms of Adiponectin exhibit distinct tissue specific and gender specific profiles and activities (12, 14).

References

  1. Lara-Castro, C. et al. (2007) Curr. Opin. Lipidol. 18:263.
  2. Tilg, H. and A.R. Moschen (2006) Nat. Rev. Immunol. 6:772.
  3. Fantuzzi, G. (2008) J. Allergy Clin. Immunol. 121:326.
  4. Yamauchi, T. et al. (2007) Nat. Med. 13:332.
  5. Takemura, Y. et al. (2007) J. Clin. Invest. 117:375.
  6. Hug, C. et al. (2004) Proc. Natl. Acad. Sci. 101:10308.
  7. Wang, Y. et al. (2005) J. Biol. Chem. 280:18341.
  8. Maeda, K. et al. (1996) Biochem. Biophys. Res. Commun. 221:286.
  9. Waki, H. et al. (2005) Endocrinology 146:790.
  10. Waki, H. et al. (2003) J. Biol. Chem. 278:40352.
  11. Tsao, T.S. et al. (2003) J. Biol. Chem. 278:50810.
  12. Wang, Y. et al. (2008) Biochem. J. 409:623.
  13. Wang, H. et al. (2006) J. Biol. Chem. 281:16391.
  14. Pajvani, U.B. et al. (2003) J. Biol. Chem. 278:9073.

Alternate Names

Acrp30, AdipoQ, ApM1, GBP28

Entrez Gene IDs

9370 (Human); 11450 (Mouse); 246253 (Rat)

Gene Symbol

ADIPOQ

UniProt

Q15848

Additional Adiponectin/Acrp30 Products

Product Documents for Human Adiponectin/Acrp30 Antibody (553517)

Certificate of Analysis

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Product Specific Notices for Human Adiponectin/Acrp30 Antibody (553517)

For research use only

Citations for Human Adiponectin/Acrp30 Antibody (553517)

Customer Reviews for Human Adiponectin/Acrp30 Antibody (553517) (1)

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  • Human Adiponectin/Acrp30 Antibody
    Name: Sijia He
    Application: Western Blot
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 11/01/2018
    Tested this antibody with western blot, it recognizes the correct band size, and the signal is strong, works perfect.
    Human Adiponectin/Acrp30 Antibody (553517) MAB10652

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