Human Aldo-keto Reductase 1C3/AKR1C3 Antibody

R&D Systems | Catalog # AF7678

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Aldo-keto Reductase 1C3/AKR1C3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Aldo-keto Reductase 1C3/AKR1C3
Met1-Tyr323
Accession # P42330

Specificity

Detects human Aldo-keto Reductase 1C3/AKR1C3 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) AKR1C1 is observed, and approximately 25% cross-reactivity with rhAKR1C4 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Aldo-keto Reductase 1C3/AKR1C3 Antibody

Detection of Human Aldo-keto Reductase 1C3/AKR1C3 antibody by Western Blot.

Detection of Human Aldo-keto Reductase 1C3/AKR1C3 by Western Blot.

Western blot shows lysates of Huh-7 human hepatoma cell line, A549 human lung carcinoma cell line, and human liver tissue. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human Aldo-keto Reductase 1C3/AKR1C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7678) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Aldo-keto Reductase 1C3/AKR1C3 at approximately 35-36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Aldo-keto Reductase 1C3/AKR1C3 by Simple WesternTM.

Left: Simple Western lane view shows lysates of human liver, loaded at 0.1 mg/ml. A specific band was detected for Aldo-keto Reductase 1C3/AKR1C3 at approximately 41 kDa (as indicated) using both 10 µg/ml and 50 µg/ml of Sheep Anti-Human Aldo-keto Reductase 1C3/AKR1C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7678) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016) in Milk-free Antibody Diluent (Catalog # 043-524). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Sheep Anti-Human Aldo-keto Reductase 1C3/AKR1C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7678) tested at 10 µg/ml (blue line) and 50 µg/ml (green line) in the human liver.

Applications for Human Aldo-keto Reductase 1C3/AKR1C3 Antibody

Application
Recommended Usage

Simple Western

10-50 µg/mL
Sample: Human liver tissue

Western Blot

0.1 µg/mL
Sample: Huh‑7 human hepatoma cell line, A549 human lung carcinoma cell line, and human liver tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Aldo-keto Reductase 1C3/AKR1C3

AKR1C3 (Aldo-Keto Reductase family 1 member C3; also 17-beta HSD 5, prostaglandin F synthase/PGFS and 3-alpha HSD type 2) is a 35-36 kDa member of the four gene 3-alpha HSD family, aldo-keto reductase superfamily of enzymes. It is expressed by multiple cell types, including prostate epithelium, T cells, and hepatocytes. AKR1C3 generates testosterone and progesterone, catalyzes the conversion of aldehydes and ketones into alcohols, and the mediates the reduction of prostaglandin D2 into PGF2. Human AKR1C3 is 323 amino acids (aa) in length. There are three potential isoform variants. One contains an alternative start site at Met120, a second shows a five aa substitution for aa 1-28, and a third possesses a 15 aa substitution for aa 124-323. Full-length human AKR1C3 shares 73% aa sequence identity with mouse AKR1C3.

Long Name

Aldo-keto Reductase Family 1, Member C3

Alternate Names

AKR1C3, AldoketoReductase 1C3, DD3, DDX, HA1753, HAKRB, HSD17B5, PGFS

Entrez Gene IDs

8644 (Human); 105349 (Mouse); 171516 (Rat)

Gene Symbol

AKR1C3

UniProt

P42330 (Human)

Additional Aldo-keto Reductase 1C3/AKR1C3 Products

Product Documents for Human Aldo-keto Reductase 1C3/AKR1C3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Aldo-keto Reductase 1C3/AKR1C3 Antibody

For research use only

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