Human Angiopoietin-like Protein 8/Betatrophin Antibody (910248)

R&D Systems | Catalog # MAB8548

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 910248
View all Angiopoietin-like Protein 8/Betatrophin Primary Antibodies »

Product Specifications

Immunogen

Human Angiopoietin-like Protein 8/Betatrophin synthetic peptide with sequences corresponding to the N-term region
Accession # Q6UXH0

Specificity

Detects human Angiopoietin-like Protein 8/Betatrophin in direct ELISA and Western Blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Angiopoietin-like Protein 8/Betatrophin Antibody (910248)

Angiopoietin-like Protein 8/Betatrophin antibody in Human Liver by Immunohistochemistry (IHC-P).

Angiopoietin-like Protein 8/Betatrophin in Human Liver.

Angiopoietin-like Protein 8/Betatrophin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Angiopoietin-like Protein 8/Betatrophin Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB8548) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Angiopoietin-like Protein 8/Betatrophin antibody by Western Blot.

Detection of Human Angiopoietin-like Protein 8/Betatrophin by Western Blot.

Western blot shows lysates of CHO Chinese hamster ovary cell line and HEK293 human embryonic kidney cell line either mock transfected or transfected with human Angiopoietin-like Protein 8 (ANGPTL8)/Betatrophin. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Angiopoietin-like Protein 8/Betatrophin Antigen Affinity-purified Polyclonal Antibody (Catalog # MAB8548) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Angiopoietin-like Protein 8/Betatrophin at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Angiopoietin-like Protein 8/Betatrophin antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

Angiopoietin-like Protein 8/Betatrophin in HepG2 Human Cell Line.

Angiopoietin-like Protein 8/Betatrophin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Angiopoietin-like Protein 8/Betatrophin Monoclonal Antibody (Catalog # MAB8548) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Angiopoietin-like Protein 8/Betatrophin Antibody (910248)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Western Blot

1 µg/mL
Sample: CHO Chinese hamster ovary cell line and HEK293 human embryonic kidney cell line transfected with human Angiopoietin-like Protein 8 (ANGPTL8)/Betatrophin

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-like Protein 8/Betatrophin

Betatrophin also known as C19orf80, ANGPTL8, and TD26, is a secreted hormone protein.  It is 198 amino acids (aa) in length, and shares 72% aa identity with mouse Betatrophin.  It is primarily expressed in the liver and adipose tissue and has been shown to be increased in the plasma of diabetic patients.  Additionally, Betatrophin has been demonstrated to increase the proliferation of beta cells in the pancreas of mice.

Alternate Names

Angiopoietin like Protein 8, ANGPTL8, Betatrophin, C19orf80, Gm6484, Lipasin, PRO1185, PVPA599, RIFL, TD26

Entrez Gene IDs

55908 (Human); 624219 (Mouse); 100361444 (Rat)

Gene Symbol

ANGPTL8

UniProt

Q6UXH0

Additional Angiopoietin-like Protein 8/Betatrophin Products

Product Documents for Human Angiopoietin-like Protein 8/Betatrophin Antibody (910248)

Certificate of Analysis

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Product Specific Notices for Human Angiopoietin-like Protein 8/Betatrophin Antibody (910248)

For research use only

Citations for Human Angiopoietin-like Protein 8/Betatrophin Antibody (910248)

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Protocols

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