Human Apolipoprotein B/ApoB Antibody (369717)

R&D Systems | Catalog # MAB4124

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Western Blot, Flow Cytometry, ELISA Capture

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 369717
View all Apolipoprotein B/ApoB Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ApoB
Met1206-Asp1413
Accession # NP_000375

Specificity

Detects human Apolipoprotein B in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Apolipoprotein B/ApoB Antibody (369717)

Detection of Human Apolipoprotein B/ApoB antibody by Western Blot.

Detection of Human Apolipoprotein B/ApoB by Western Blot.

Western blot shows human plasma. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Apolipoprotein B/ApoB Monoclonal Antibody (Catalog # MAB4124) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Apolipoprotein B/ApoB at approximately 500 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Apolipoprotein B/ApoB antibody in Human Liver by Immunohistochemistry (IHC-P).

Apolipoprotein B/ApoB in Human Liver.

Apolipoprotein B/ApoB was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Apolipoprotein B/ApoB Monoclonal Antibody (Catalog # MAB4124) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human Apolipoprotein B/ApoB by Simple WesternTM.

Simple Western shows lysates of human plasma, loaded at 1:50. A specific band was detected for Apolipoprotein B/ApoB at approximately 333 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human Apolipoprotein B/ApoB Monoclonal Antibody (Catalog # MAB4124). This experiment was conducted under reducing conditions and using the 66-440kDa separation system.

Applications for Human Apolipoprotein B/ApoB Antibody (369717)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Simple Western

5 µg/mL
Sample: Human plasma

Western Blot

2 µg/mL
Sample: Human plasma

Reviewed Applications

Read 1 review rated 5 using MAB4124 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Apolipoprotein B/ApoB

Human ApoB (Apolipoprotein B-100) is a 550 kDa, secreted, palmitoylated glycoprotein that is part of LDL and VLDL particles. It is made by liver and is 4536 amino acids (aa) in length. It binds LDL to the ApoB/E receptor. Multiple forms of ApoB-100 exist; ApoB-25, 26, 29, 37, 39, 48, and 74. The numbers reflect each molecule’s predicted MW as a percentage of ApoB-100 (512 kDa). Amino acids 1206-1414 of the precursor were used for immunization. Based on likelihood of secretion, this antibody may recognize B26 (1297 aa), B37 (1728 aa), B39 (1799 aa), and B48 (2152 aa). Over the immunization polypeptide range, human ApoB-100 is 80% and 82% aa identical to mouse and dog ApoB-100, respectively.

Alternate Names

APOB, ApoB-100, ApoB-48, FLDB, LDLCQ4

Entrez Gene IDs

338 (Human); 238055 (Mouse); 54225 (Rat)

Gene Symbol

APOB

UniProt

NP_000375

Additional Apolipoprotein B/ApoB Products

Product Documents for Human Apolipoprotein B/ApoB Antibody (369717)

Certificate of Analysis

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Product Specific Notices for Human Apolipoprotein B/ApoB Antibody (369717)

For research use only

Related Research Areas

Obesity

Citations for Human Apolipoprotein B/ApoB Antibody (369717)

Customer Reviews for Human Apolipoprotein B/ApoB Antibody (369717) (1)

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  • Human Apolipoprotein B/ApoB Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Liver tissue
    Species: Human
    Verified Customer | Posted 11/17/2021
    Human Apolipoprotein B/ApoB Antibody (369717) MAB4124

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Protocols

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