Axin-2, also known as Conductin, is a 95 kDa cytoplasmic protein that inhibits Wnt signaling and promotes epithelial-mesenchymal transition. Axin 2 binds to beta -catenin, glycogen synthase kinase-3 (GSK-3 beta ), and the tumor suppressor APC. Loss of Axin-2 function is linked to carcinogenesis as well as abnormal bone and tooth development. Within aa 550-760, human Axin-2 shares 82% aa sequence identity with mouse and rat Axin-2.
Human Axin-2 Antibody (354214)
R&D Systems | Catalog # MAB6078
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry
Cited:
Immunohistochemistry, ELISA Capture
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 354214
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Product Specifications
Immunogen
E. coli-derived recombinant human Axin-2
Tyr550-Gln760
Accession # Q9Y2T1
Tyr550-Gln760
Accession # Q9Y2T1
Specificity
Detects human Axin-2 in direct ELISAs. In this format, no cross-reactivity with recombinant human Axin-1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Axin-2 Antibody (354214)
Axin‑2 in SW480 Human Cell Line.
Axin-2 was detected in immersion fixed SW480 human colorectal adenocarcinoma cell line using Mouse Anti-Human Axin-2 Monoclonal Antibody (Catalog # MAB6078) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Axin-2 Antibody (354214)
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion-fixed SW480 human colorectal adenocarcinoma cell line
Sample: Immersion-fixed SW480 human colorectal adenocarcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Axin-2
Long Name
Axis Inhibitor 2
Alternate Names
AXIL, Axin2, Conductin, MGC10366, MGC126582
Entrez Gene IDs
8313 (Human)
Gene Symbol
AXIN2
UniProt
Additional Axin-2 Products
Product Documents for Human Axin-2 Antibody (354214)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Axin-2 Antibody (354214)
For research use only
Related Research Areas
Citations for Human Axin-2 Antibody (354214)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
