Human Bcl-9 Antibody (417514)

R&D Systems | Catalog # MAB3996

R&D Systems
Discontinued Product
MAB3996 has been discontinued. View all Bcl-9 products.

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 417514
View all Bcl-9 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Bcl-9
Met1009-Gly1328
Accession # O00512

Specificity

Detects endogenous human Bcl-9 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Bcl-9 Antibody (417514)

Detection of Human Bcl‑9 antibody by Western Blot.

Detection of Human Bcl‑9 by Western Blot.

Western blot shows lysates of HCT-116 human colorectal carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Human Bcl-9 Monoclonal Antibody (Catalog # MAB3996) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Bcl-9 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Bcl-9 Antibody (417514)

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: HCT-116 human colorectal carcinoma cell line and K562 human chronic myelogenous leukemia cell line

Reviewed Applications

Read 2 reviews rated 4 using MAB3996 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Bcl-9

Bcl-9 (B-cell lymphoma 9; also Protein legless homolog) is a 149 kDa transcriptional regulator that belongs to the Bcl-9 family of proteins. It is expressed in multiple tissues and serves to recruit Pygopus to the Wnt-pathway beta -catenin-TCF complex in the nucleus. Bcl-9 and Bcl-9-2 are considered evolutionary duplicates of Legless that perform the same task with different regulatory mechanisms. Human Bcl-9 is 1426 amino acids (aa) in length. It contains one phosphothreonine and three phosphoserine sites, two poly-Pro regions (aa 514‑517 and 970‑973), and one poly-Ala segment (aa 900‑903). There is one potential alternate start site at Met27, and a variant isoform exists that shows a four aa substitution for aa 1391‑1426. Over aa 1009‑1328, human Bcl-9 is 96% aa identical to mouse Bcl-9. B cell cancers often have translocations at the 3'UTR region of the Bcl-9 gene.

Long Name

B Cell Lymphoma/Leukemia 9, Nuclear Co-factor of beta-Catenin Signaling

Alternate Names

Bcl9, BCL9L, LGS

Entrez Gene IDs

607 (Human); 77578 (Mouse); 310704 (Rat)

Gene Symbol

BCL9

UniProt

O00512

Additional Bcl-9 Products

Product Documents for Human Bcl-9 Antibody (417514)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Bcl-9 Antibody (417514)

For research use only

Customer Reviews for Human Bcl-9 Antibody (417514) (2)

4 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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  • Human Bcl-9 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/06/2019
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human Bcl-9 Antibody (417514) MAB3996
  • Human Bcl-9 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Plasma
    Species: Human
    Verified Customer | Posted 11/10/2018

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