Human beta-Actin Antibody (937210)

R&D Systems | Catalog # MAB8969

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 937210
View all beta-Actin Primary Antibodies »

Product Specifications

Immunogen

Peptide containing a sequence at the N-terminus of human beta ‑Actin
Accession # P60709

Specificity

Detects human beta ‑Actin in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human beta-Actin Antibody (937210)

beta -Actin antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

beta ‑Actin in HeLa Human Cell Line.

beta -Actin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human beta -Actin Monoclonal Antibody (Catalog # MAB8969) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human beta-Actin Antibody (937210)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB8969 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: beta-Actin

Actin makes up the most abundant protein in many eukaryotic cell types. It polymerizes to form microfilaments that have an array of functions, including regulating contractility, motility, cytokinesis, phagocytosis, adhesion, cell morphology, and providing structural support. At least six types of Actin, falling into three categories, exist in higher eukaryotes. The three types, separated based on isoelectric point, include alpha ( alpha -Skeletal, alpha -Cardiac, alpha -Smooth Muscle), beta ( beta -Non-muscle) and  gamma
( gamma -Smooth Muscle, gamma -non-muscle) isoforms.  Non-muscle beta - and gamma -actin are also known as cytoplasmic actin.

Alternate Names

ACTB, betaActin

Entrez Gene IDs

60 (Human); 11461 (Mouse); 81822 (Rat)

Gene Symbol

ACTB

UniProt

P60709

Additional beta-Actin Products

Product Documents for Human beta-Actin Antibody (937210)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human beta-Actin Antibody (937210)

For research use only

Citations for Human beta-Actin Antibody (937210)

Customer Reviews for Human beta-Actin Antibody (937210) (1)

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  • Human beta -Actin Antibody
    Name: Christopher England
    Application: Western Blot
    Sample Tested: lung cancer cells
    Species: uman pancreatic cancer cells and uman cancer cells in mouse lung (xenograft)
    Verified Customer | Posted 12/05/2016
    CTLA-4 expression examined in three human lung cancer cell lines. Only one of the cell lines showed expression, yet B-actin was visible at equal concentrations in each lane.
    After reconstitution at 0.5 mg/mL, we used a ratio of 1:200 for the Western blot.
    Human beta-Actin Antibody (937210) MAB8969

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FAQs for Human beta-Actin Antibody (937210)

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  • Q: Does this Beta-Actin antibody come in lyophilized form?

    A: Yes, we carry this beta-actin antibody in lyophilized format.

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